目的 建立绵茵陈中绿原酸的含量测定方法并采用薄层色谱 - 生物自显影法研究绵茵陈提取液的抗氧化活性。 方法 薄层 扫描法测定绵茵陈提取液中绿原酸的含量,随后用 二苯代苦味肼基自由基 溶液显色,双波长扫描,获得其抗氧化成分的峰面积。 结果 常规薄层扫描与生物自显影扫描测定得到的 绿原酸 含量具有一致性, 由 生物自显影扫描法测得 色谱峰 总积分值明显大于绿原酸单峰面积,表明 绵茵陈中除了绿原酸外,还存在其它抗氧化能力较强的活性成分。 结论 以 DPPH 作显色剂,双波长扫描,可利用 TLC- 生物自显影技术对天然提取物中具有自由基清除及抗氧化活性的活性成分进行快速筛选与评价 。
Abstract
OBJECTIVE To establish a method to determine the contents of chlorogenic acid in Herba Artemisiae scopariae extracts and evaluate its antioxidant capacity by TLC-bioautography. METHODS Single wavelength TLC-scanning method was used to determinate the concertration of chlorogenic acid. Then, the chromatograms were obtained after spraying with 1, 1-diphenyl-2-picrylhydrazyl free radicals (DPPH) solution and the peak areas of the spots on TLC were calculated to evaluate the antioxidant capacity of Herba Artemisiae scopariae extracts. RESULTS The contents of chlorogenic acid determined by TLC-bioautography is consistent with routine method,and the total integral value measured by bioautography is significantly higher than the single peak area of chlorogenic acid. The results indicated Herba Artemisiae scopariae had many antioxidant active ingredients besides chlorogenic acid. CONCLUSION With DPPH as color developing reagent and dual wavelength scanning, TLC-bioautography can be used for fast selecting and evaluating the antioxidant capacity of traditional Chinese medicines
关键词
绵茵陈 /
绿原酸 /
薄层色谱 - 生物自显影 /
抗氧化 /
二苯代苦味肼基自由基
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Key words
herba Artemisiae scopariae /
chlorogenic acid /
TLC-bioautography /
antioxidant /
DPPH
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参考文献
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( 收稿日期 : 2009-01-10 )
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脚注
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