目的 优化 D101 大孔 树脂分离 连翘酯苷 的条件和快速检测方法。 方法 连翘提取液经过 D101 大孔 树脂吸附后 ， 以不同浓度乙醇溶液洗脱 ， 采用 HPLC 测定连翘酯苷的含量，并以 测定值作参比，判定紫外扫描跟踪检测方法的可靠性。一次正交考察树脂用量、 连翘提取液 浓度、上样时间等因素对树脂吸附率的影响 ； 二次正交考察乙醇浓度、洗脱液用量、洗脱速度对连翘酯苷纯度及树脂解吸率的影响 ， 并绘制 连翘酯苷 的洗脱曲线。 结果 树脂用量对 连翘酯苷 吸附量影响极显著 ， 最佳吸附条件为 A2B 3C 2D2 。 最佳解吸条件根据考察指标不同有所差异 ， 以纯度为指标时最佳条件为 a2b 1c 1 ； 以得率为指标时最佳条件为 a3b 1c 3 。洗脱液中含连翘酯苷时， 紫外扫描图谱和连翘酯苷对照品的图谱基本一致。 结论 D101 大孔 树脂作为分离 连翘酯苷 较好的分离材料 ，对其进行 优化的分离工艺可行；应用紫外扫描可快捷检测连翘酯苷的洗脱情况。
Abstract
OBJECTIVE To research the optimized procedure of forsythiaside separation by D101 macroporous resin and detect it by ultraviolet spectrophotometry. METHODS The extraction solution of forsythia suspensa was absorbed by macroporous resin, and then the resin was eluted with different concentration alcohol. The contents of forsythiaside were determined by HPLC. HPLC assay was a reference method for the reliability of the ultraviolet spectrophotometry real-time tracking detection. The first orthogonal test investigated the effect of resin quantity, extract concentration and time on the adsorption of forsythiaside. The second orthogonal test investigated the effect of ethanol percentage, elution liquid quantity and flow rate on elution. The elution curve was obtained. RESULTS Resin quantity had significant influence on adsorption and the optimum adsorption condition were A2B3C2D2. The optimized elution condition was different according to purity or yield as index. The optimum condition for purity were a2b1c1. The optimum conditions for yield were a3b1c3. CONCLUSION Ultraviolet spectrophotometry can be used in real-time tracking detection of forsythiaside. D101 resin is suitable for the separation of forsythiaside. The optimized procedure is practical. The method of ultraviolet spectrophotometry detection with separation of forsythiaside by D101 macroporous resin is rapid.
关键词
D101 大孔树脂 /
连翘酯苷
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Key words
D101 macroporous resin /
forsythiaside /
dectection
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参考文献
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（ 收稿日期 ： 2009-05-07 ）
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