摘要
目的对纳米粒毛细管电泳在化合物分离研究中的应用作一综述。方法在引用大量文献的基础上,论述纳米粒在毛细管电泳系统的作用机制及在毛细管电泳中纳米粒应具备的特性,并分别介绍了聚合物纳米粒、分子印迹聚合物纳米粒、树枝状聚合物纳米粒、金纳米粒、二氧化硅纳米粒在毛细管电泳中的应用研究,最后指出了该技术的优点及局限性。结果毛细管电泳中,纳米粒的引入能显著提高分离的选择性和分离效率,并能实现与质谱的联用。结论纳米粒毛细管电泳系统用以分离各种化合物显示出了巨大的发展潜力。
关键词
毛细管电泳 /
假固定相
{{custom_keyword}} /
刘艳华;王永军;孙进;何仲贵.
纳米粒在毛细管电泳中的应用[J]. 中国药学杂志, 2009, 44(12): 881-884
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
参考文献
[1] WANG X K,HE Y Z,QIAN L L. Determination of polyphenol components in herbal medicines by micellar electrokinetic capillary chromatography with Tween 20[J]. Talanta,2007,74(1):1-6.
[2] CHU B L,GUO B Y,ZUO H J,et al. Simultaneous enantioseparation of antiparkinsonian medication rotigotineand related chiral impurities by capillary zone electrophoresis using dual cyclodextrin system[J]. J Pharm Biomed Anal,2008,46(5):854-859.
[3] WANG Y J,SUN J,HE Z G. Liposome electrokinetic chromatography and its application in evaluating drug-membrane interaction[J]. Acta Pharm Sin(药学学报),2006,41(7):583-588.
[4] PUIG P,BORRULL F,AGUILAR C,et al. Sample stacking for the analysis of penicillins by microemulsion electrokinetic capillary chromatography[J]. J Chromatogr B,2006,831(1-2):196-204.
[5] WALLINGFORD R A,EWING A G. Capillary Electrophoresis[J]. Adv Chromatogr,1989,29(1):1-67.
[6] AMINI A,PAULSEN U,WESTRLUND D. Principle and applications of the partical filling technique in capillary electrophoresis[J]. Chromatographia,1999,50(7-8):479-483.
[7] B?CHMANN K,G?TTLICHER B,HAAG I,et al.Capillary electrokinetic chromatography with a separation of chromatography particles[J]. J Chromatogr A,1994,688(1-2):283-292.
[8] NILSSON C,VIBERG P,NILSSON S,et al. Nanoparticle-based continuous full filling capillary electrochromatography/electrospray ionization-mass spectrometry for separation of neutral compounds[J]. Anal Chem,2006,78(17):6088-6095.
[9] VIBERG P,SPEGEL P,NILSSON J. Reversed phase continuous full filling CEC-ESI-MS[J]. Chromatographia,2007,65(5-6):291-297.
[10] VIBERG P,PETERSSON P,SPEGEL P. Nanoparticles as pseudostationary phase in capillary electrochromatography/ESI-MS [J]. Anal Chem,2002,74(18):4595-4601.
[11] NEIMAN B,GRUSHKA E,LEV O. Use of gold nanoparticles to enhance capillary electrophoresis[J]. J Appl Phys,2001,89(9) :4548-4553.
[12] NEIMAN B,GRUSHKA E,LEV O. Use of gold nanoparticles to enhance capillary electrophoresis[J]. Anal Chem,2001,73(21):5220-5227.
[13] NILSSON C,NILSSON S. Nanoparticle-based pseudostationary phases in capillary electrochromatography[J]. Electrophoresis,2006,27(1):76-83.
[14] G?TTLICHER B, B?CHMANN K. Application of particles as pseudo-stationary phases in electrokinetic chromatography[J]. J Chromatogr A,1997,780(1-2):63-73.
[15] VIBERG P,SPEGEL P,CARLSTEDT J,et al. Continuous full filling capillary electrochromatography:Chromatographic performance and reproducibility[J]. J Chromatogr A, 2007,1154(1-2):386-389.
[16] NILSSON C,BIRNBAUM S,NILSSON S.Use of nanoparticles in capillary and microchip electrochromatography[J]. J Chromatogr A,2007,1168(1-2):212-224.
[17] BREADMORE M C,MACKA M,HADDAD P R.Manipulation of separation selectivity for alkali metals and ammonium in ion-exchange capillary electrochromatography using a suspension of cation exchange particles in the electrolyte as a pseudostationary phase[J]. Electrophoresis,1999,20(10):1987-1992.
[18] NA N,HU Y,OUYANG J,et al.Use of polystyrene nanoparticles to enhance enantiomeric separation of propranolol by capillary electrophoresis with Hp-beta-CD as chiral selector[J]. Anal Chim Acta,2004,527(2):139-147.
[19] DE BOER T,MOL R,DE ZEEUW R,et al. Spherical molecularly imprinted polymer particles:A promising tool for molecular recognition in capillary electrokinetic separations[J]. Electrophoresis,2002,23(9):1296-1300.
[20] SCHWEITZ L,SPéGEL P,NILSSON S.Molecularly imprinted microparticles for capillary electrochromatographic enantiomer separation of propranolol[J]. Analyst, 2000,125(11):1899-1901.
[21] WALSHE M,GARCIA E,HOWARTH J,et al. Separation of enantiomers of propranolol by incorporation of molecularly imprinted polymer particles as chiral selectors in capillary electrophoresis[J]. Anal Chem,1997,34(2):119-122.
[22] SCHWEITZ L,SPéGEL P, NILSSON S.Molecularly imprinted microparticles for capillary electrochromatography:Studies on microparticle synthesis and electrolyte composition[J]. Electrophoresis,2001,22(17):3833-3841.
[23] SHI X Y,ISTVAN J,MAJOROS I J,et al. Capillary electrophoresis of poly(amidoamine) dendrimers:from simple derivatives to complex multifunctional medical nanodevices[J]. Mol Pharm,2005,2(4):278-294.
[24] GRAY A L,HSU J T.Novel sulfonic acid-modified starburst dendrimer used as a pseudostationary phase in electrokinetic chromatography[J]. J Chromatogr A,1998,824(1):119-124.
[25] STATHAKIS C,ARRIAGA E A,DOVICHI N J.Protein profiling employing capillary electrophoresis with dendrimers as pseudostationary phase media[J]. J Chromatogr A,1998,817(1-2):233-238.
[26] HUANG M F,KUO Y C,HUANG C C,et al. Separation of long double-stranded DNA by nanoparticle-filled capillary electrophoresis[J]. Anal Chem,2004,76(1):192-196.
[27] G?TTLICHER B, B?CHMANN K. Investigation of the separation efficiency of hydrophobic compounds in suspension electrokinetic chromatography[J]. Electrophoresis,2006,27(2):76-83.
[28] B?CHMANN K,G?TTLICHER B. New particles as pseudostationary phase for electrokinetic chromatography[J]. Chromatographia,1997,45(17):249-253.
[29] FUJIMOTO C,MURANAKA Y. Electrokinetic chromatography using nanometer-sized silica particles as the dynamic stationary phase[J]. J High Resol Chromatogr,1997,20(7):400-402.
[30] NEIMAN B,GRUSHKA E,GUN J,et al. Organically modified silica sol-mediated capillary electrophoresis[J]. Anal Chem,2002,74(14):3484-3491.
[31] CHRISTOPHER P,JONATHAN P. Recent progress in the use of soluble ionic polymers as pseudostationary phase for electrokinetic chromatography[J]. Electrophoresis,2004,25(23-24):4086-4094.
[32] WU X H,ZOU Q G,XIANG B R. Application of molecular imprinting to chrial separation in capillary electrochromatography[J]. Prog Pharm Sci(药学进展),2004,28(5):222-226.
[33] YAO S W,ZOU Y,ZHANG W G. Research progress of the properties,preparation and application of Au nanoparticles[J]. Chem Ind Eng Prog(化工进展),2007,26(3):310-319.
[34] ZHOU D,WANG Y M. Progress in effect of additives in DNA separation by capillary electrophoresis [J].Polym Bull(高分子通报),2006,10(10):76-81.
[35] YU C J,SU C L,TSENG W L. Separation of acidic and basic protein by nanoparticle-filled capillary electrophoresis[J].Anal Chem,2006,78(23):8004-8010.
{{custom_fnGroup.title_cn}}
脚注
{{custom_fn.content}}