目的建立以三萜类化合物紫菀酮为内标物,同时测定枸骨叶药材中熊果酸和羽扇豆醇含量的反相高效液相色谱(RP-HPLC)分析方法。方法色谱柱:Agilent Zorbax ODS(4.6 mm×150 mm,5μm),流动相:以乙腈-0.1%磷酸水,梯度洗脱;流速:1.0 mL·min-1;检测波长:210 nm。结果10个不同收集地的枸骨叶中熊果酸和羽扇豆醇的含量分别在1.23%～1.63%和0.59%～0.95%之间。结论该法快速、灵敏度高、重复性好,可用于测定枸骨叶中主要三萜类化合物熊果酸和羽扇豆醇的含量测定。
Abstract
OBJECTIVE To develop a quantitative reversed phase high performance liquid chromatography method using diode array detector for the determination of ursolic acid and lupeol in leaves of Ilex cornuta.METHODS An Agilent Zorbax ODS column was used with a gradient elution composed of acetonitrile and 0.1%phosphoric acid solution at a flow rate of 1.0 mL·min-1,Shionone was used as the internal standard.RESULTS This method was validated in term of linearity,accuracy, precision,reproducibility and stability.The method was applied to analyze leaves of Ilex cornuta from 10 different locations.As a result,the contents of ursolic acid and lupeol were 1.23%-1.63%and 0.59%-0.95%,respectively.CONCLUSION The established method was proved to be simple,reproducible,accurate,and can be used to evaluate the quality of leaves of Ilex cornuta.
关键词
枸骨叶 /
熊果酸 /
羽扇豆醇 /
反相高效液相色谱法 /
内标法
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Key words
Ilex cornuta /
ursolic acid /
lupeol /
RP-HPLC /
internal standard method
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参考文献
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脚注
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