摘要
目的研究超声波与丙醇-硫酸铵双水相耦合从3种提取分离黄酮的工艺条件及参数及提取物抗氧化活性。方法以Al(NO3)3-NaNO2分光光度法测定总黄酮,研究丙醇-硫酸铵双水相形成条件及超声提取条件对总黄酮提取率的影响,优化提取条件,以Fe2+为脂质过氧化促进剂,以卵磷脂脂质体为模型研究提取物抗氧化活性。结果对3种中药中总黄酮的提取率在2.7%～6.12%之间,与回流提取法相近,而提取物中总黄酮含量为17.2%～21.1%,明显高于回流提取法,提取物对脂质过氧化最大抑制率在70.1%～74.4%之间。结论方法可有效地从中药中提取黄酮,提取物对脂质过氧化具有良好的抑制作用,方法操作简便、条件温和,环境友好,适于中药中黄酮的提取。
Abstract
OBJECTIVE To research the extraction and separation of flavonoid in three Chinese materia medics Elshola blanda,Ginkgo biloba Linn and Lysimachia christinae Hance by ultrasonic extraction technology coupling with propyl-alcohol ammonium sulfateaqueous aqueous two-phase separation and determine the antioxidantion activities of extractives.METHODS The contents of general flavonoids were determined by Al(NO3)3-NaNO2 spectrophotometric methods,and the inhibition rate of lipid peroxidation(LPO) induced by·OH free radical generation system was used to evaluate the antioxidantion activities of extractives. RESULTS The extraction rates of general flavonoids were 2.7%-6.12%for three kinds of Chinese materia medics in the optimum extracting conditions,and the general flavonoids contents in extractives were 17.3%-21.1%,much higher than that of reflux extraction of solvent,and the maxium LPO inhibit rates of the extractives of three kinds of Chinese materia medics were between 70.1%-74.4%.CONCLUSION The proposed method could extracte general flavonoids from Chinese materia medics effectively. The extractiuon conditions were mild and friendly environmentally.The extractives could singificantly inhibit LPO induced by·OH free radical.
关键词
超声波提取 /
双水相分离 /
中药 /
黄酮
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Key words
ultrasonic extraction /
aqueous two-phase separation /
Chinese materia medic /
flavonoid
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高云涛;李羚;戴建辉;贝玉祥;郭英.
从鸡肝散、银杏叶和金钱草中提取分离黄酮及抗氧化活性研究[J]. 中国药学杂志, 2009, 44(10): 736-739
GO Yun-to;LI Ling;DI Jin-hui;EI Yu-xing;GUO Ying.
Extraction of Flavonoid from Chinese Materia Medics by Ultrasonic Extraction Coupling with Propyl-alcohol Ammonium Sulfate Aqueous Two-phase Separation and Its Antioxidantion of Extractives [J]. Chinese Pharmaceutical Journal, 2009, 44(10): 736-739
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参考文献
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脚注
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基金
云南省社会发展科技计划资助项目(2007B148M);云南省教育厅科学基金资助项目(06Y195C);国家民委-教育部共建民族药资源化学重点实验室开放基金资助项目(MJY07010)
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