目的建立同时测定伤科七味片中士的宁和马钱子碱含量的方法。方法采用Agilent ZORBAXSB C₁₈柱(4.6mm×250mm,5μm),流动相:乙腈-0.4%磷酸水溶液(用三乙胺调节pH值为3.50)(10∶90),流速1.0mL·min^-1,检测波长:254nm。结果士的宁和马钱子碱的线性范围分别为0.0096～0.0960g·L^-1,0.0049～0.0490g·L^-1;平均加样回收率(n=6)分别为0.38%,0.74%。结论该方法简便快速、结果准确可靠,适用于伤科七味片的质量控制。

OBJECTIVE To establish a HPLC method for the determination of strychnine and brucine in Shangkeqiwei tablets.METHODS The sample was analyzed on an Agilent ZORBAX SB C₁₈ column(4.6 mm×250 mm,5 μm) with mobile phase consisting of 0.4% phosphoric acid and acetonitrile(pH adjusted to 3.50 with triethylamine)(90:10)at the flow rate of 1.0 mL·min^-1 and the detection wavelength was 254 nm.RESULTS The calibration curves of strychnine and brucine were in good linearity over the ranges of 0.009 6～0.096 0 g·L^-1 and 0.004 9～0.049 0 g·L^-1,and the average recoveries(n=6)were 0.38% and 0.74%,respectively。CONCLUSION The method is simple,rapid and accurate and can be used for quality control of Shangkeqiwei tablets.