摘要
目的优选芎汤醇提的最佳工艺条件。方法采用高效液相色谱指纹图谱技术,以阿魏酸含量、高效液相色谱指纹图谱特征峰总面积以及干浸膏得率为综合评判指标,通过L9(3)4正交实验设计,考察乙醇浓度、乙醇用量、回流时间和回流次数等因素对提取工艺的影响。结果醇提最佳工艺条件为加12倍量体积分数70%乙醇提取3次,每次1.5h。结论该醇提工艺合理可行,优于传统提取方法。
Abstract
OBJECTIVE To optimize the ethanol extraction process and condition of Xiongqiong decoction.METHODS Using high performance liquid chromatography(HPLC) fingerprint technology,the effects of ethanol concentration,ethanol amount,extraction time and the number of extraction times on the yield of extract were evaluated by orthogonal test design with multi-index of the content of ferulic acid,the total area of characteristic peaks of HPLC and the yield of powdered extract.RESULTS The optimum condition of the ethanol extraction was obtained.The raw material amount was extracted with 12 volumes of 70% ethanol three times for 1.5 h per extraction process.CONCLUSION The optimized extraction method was more effective than the traditional extraction method and can be used for the extraction process of Xiongqiong decoction.
关键词
芎汤 /
醇提工艺 /
阿魏酸 /
高效液相色谱指纹图谱 /
正交实验设计
{{custom_keyword}} /
Key words
Xiongqiong decoction /
ethanol extraction /
ferulic acid /
HPLC fingerprint /
orthogonal test design
{{custom_keyword}} /
容蓉;陈明强;吕青涛;孙秀梅;杨勇.
正交实验结合高效液相色谱指纹图谱优选芎汤醇提工艺[J]. 中国药学杂志, 2009, 44(08): 580-584
RONG Rong;CHEN Ming-qing;Lü Qing-to;SUN Xiu-mei;YNG Yong.
Optimization of Ethanol Extraction Process and Condition For Xiongqiong Decoction by Orthogonal Test Design and HPLC [J]. Chinese Pharmaceutical Journal, 2009, 44(08): 580-584
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
参考文献
[1] TIAN D H. Practical Pair Formula of Traditional Chinese Medicine (实用中医对药方) [M]. Beijing：People’s Medical Publishing House，2000：567.
[2] HU Y Y，XU X Y. Chemical and pharmaceutical research development of ferulic acid [J]. Chin Tradit Pat Med(中成药)，2006，28(10)：253-255.
[3] SUN G X，BI K S. Effect of fingerprintology of traditional Chinese medicine (TCM) in the innovative development of TCM [J]. Chin J Chromatogr (色谱)，2008，26(2)：172-179.
[4] HU Z J. The application of SPSS11.5 in orthogonal test design [J]. Med Inf(医学信息)，2007，20(5)：737-740.
[5] ZHANG C H，YAN Y L. Mathematical Statistics in Medicine (医药数理统计) [M]. Beijing：Science Press，2001：212.
[6] HAN B，CHEN W，HAN C M. The application of high throughput screening in studying of TCM [J]. J Jiangxi Univ Tradit Chin Med (江西中医学院学报)，2005，17(5)：36-38.
{{custom_fnGroup.title_cn}}
脚注
{{custom_fn.content}}
基金
中国博士后基金资助课题(20060400992);山东省中医管理局课题(2004-23)
{{custom_fund}}
