目的对柽柳(Tamarix chinensis Lour.)抗肿瘤活性成分进行分离和鉴定。方法使用硅胶、SephadexLH-20、ODS和HPLC分离、纯化,利用NMR技术确定结构。采用MTT法和SRB法测定了所得化合物的抗肿瘤活性。结果分离鉴定了6个甾体类化合物和7个黄酮类化合物,分别为β-谷甾醇(β-sitosterol,1)、豆甾-4-烯-3,6-二酮(stigmast-4-ene-3,6-dione,2)、麦角甾-4,24(28)-二烯-3-酮[ergosta-4,24(28)-diene-3-one,3]、豆甾烷-3,6-二酮(stigmast-4-ene-3,6-dione,4)、豆甾-4-烯-3-酮(sitosterone,5)、胆甾醇(cholesterol,6)、5-羟基-7,4′-二甲氧基黄酮(5-hydroxy-7,4′-dimethoxyflavone,7)、山柰酚(kaempferol,8)、山柰酚-4′-甲醚(4′-methylkaempfero,9)、山柰酚-7-甲醚(7-methylkaempfero,10)、山柰酚-4′,7-二甲醚(4′,7-dimethylkaempfero,11)、槲皮素(quercetin,12)、槲皮素-3′,4′-二甲醚(3′,4′-dimethylquercetin,13)。结论化合物2,3,4,5和7首次从柽柳属中分离得到。体外抗肿瘤活性实验显示,在50mg·L-1下,化合物2,3,4和5对人肺癌细胞A-549显示有较强的细胞毒活性。
Abstract
OBJECTIVE To investigate the chemical constituents with antitumor activity from Tamarix chinensis Lour..METHODS Column chromatographic methods were used to isolate the chemical constituents.NMR methods were employed for their structural elucidation.The effects of constituents on tumor cells were tested by MTT and SRB methods.RESULTS 6 Steroids and 7 flavonoids were isolated and identified as β-sitosterol(1),stigmast-4-ene-3,6-dione(2),ergosta-4,24(28)-diene-3-one(3),stigmast-4-ene-3,6-dione(4),sitosterone(5),cholesterol(6),5-hydroxy-7,4'-dimethoxyflavone(7),kaempferol(8),4'-methylkaempfero(9),7-methylkaempferol(10),4',7-dimethylkaempfero(11),quercetin(12) and 3',4'-dimethylquercetin(13),respectively.CONCLUSION 6 Steroids and 7 flavonoids were isolated from Tamarix chinensis Lour.and compound 2,3,4,5 and 7 were isolated from this genus for the first time.Compound 2,3,4 and 5 show significant inhibitory effects on human lung cancer cell A-549 at the concentration of 50 mg·L-1.
关键词
柽柳 /
甾体 /
黄酮 /
抗肿瘤活性
{{custom_keyword}} /
Key words
Tamarix chinensis Lour. /
steroids /
flavonoids /
antitumor activity
{{custom_keyword}} /
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
参考文献
[1] Ch.P（2005） VolⅠ(中国药典2005年版. 一部) [S]. 2005：87.
[2] NASREEN F，NAVAID Z. Pharmacological and toxicological studies of Icterene，a herbal formulation of Tamarix pakistanica，prepared by Hamdard aboratories（Waqf) Pakistan，in rodents [J]. Proc Pakistan Congr Zool，1993，13：185-194.
[3] ZHAO R Z，SUN S Y，CHEN F K. Study on pharmacological functions of Tamarix chinensis Lour[J]. Chin Tradit Herb Drugs（中草药），1995，26（2）：85.
[4] YUAN K，LU J L，JIA A. Studies on chemical constituents of Mimosa pudica [J]. Chin Pharm J（中国药学杂志），2006，41(17)：1293-1295.
[5] WEI K，LI W，KOIKE K，et al. Complete 1H and 13C-NMR assignments of two phytosterols from roots of Piper nigrum [J]. Magn Reson Chem，2004，42(3)：355-359.
[6] LI G Q，DENG Z W，GUAN H S，et al. Steroids from the soft coral Dendronephthya sp. [J]. Steroids，2005，70（1）：13-18.
[7] KONTIZA I，ABATIS D，MALAKATE K，et al. 3-Keto steroids from the marine organisms Dendrophyllia cornigera and Cymodocea nodosa [J]. Steroids，2006，71(2)：177-181.
[8] LI J X，LI J，XU L Z，et al. Studies on chemical constituents of Cordyceps sinensis（Berk） Sacc [J]. Chin Pharm J（中国药学杂志），2003，38(7)：500-502.
[9] NAWWAR M A M，BUDDRUS J，BAUER H. Dimeric phenolic constituents from the roots of Tamarix nilotica [J]. Phytochemistry，1982，21(7)：1755-1758.
[10] QIU G H，ZUO W J，WANG J H，et al. Studies on the chemical Constituents of Ledum palustre L.[J]. China J Mod Med（中国现代医学杂志），2006，8(6)：18-21.
[11] XIAO J，WANG G L，WEI F，et al. Study of chemical constituents from Eupatorium lindleyanum DC. [J]. Chin Tradit Herb Drugs（中草药），2004，35(8)：355-356.
[12] HUANG X F，LUO J，ZHANG Y，et al. Chemical constituents of Asparagus officinalis [J]. Chin J Nat Med（中国天然药物)，2006，14(3)：181-184.
[13] DAI C，LIU P，LIU C，et al. Studies on chemical constituents from moss Rhodobryum roseum Ⅱ[J]. Chin J Nat Med（中国天然药物），2006，31(13)：1080-1082.
[14] LI Y，GUO S X，WANG C L，et al. Studies on flavonoids of Saussurea involucrata [J]. Chin Pharm J（中国药学杂志），2007，38(7)：575-577.
[15] ZHANG X Y，LING L Q，MAO Q M. Study of chemical constituents from Tamarix chinensis Lour. [J]. Chin Tradit Herb Drugs（中草药），1989，20(8)：4-5.
{{custom_fnGroup.title_cn}}
脚注
{{custom_fn.content}}
基金
国家海洋局908专项(908-01-ST12,908-02-05-04);863重点项目(2007AA091701);浙江省自然科学基金项目(Y2080579)
{{custom_fund}}
