Study on Binding of Apoprotein of Lidamycin to Cancer Cells
WANG Jian-kang1,2,MENG Ru-song1,LI Zhong-dong1
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1. General Hospital of Air Force,Beijing 100036,China;2.Institute of Medicinal Biotechnology,Academy of Medical Science & Peking Union Medical College,Beijing 100050,China
OBJECTIVE This experiment was set to study binding of the apoprotein to cancer cells and examine the correlation between the binding action and cancer biological index such as ER、P53、Bcl-2. METHODS LDP was linked to FITC, a fluorescent reagent. The FITC-LDP conjugate was incubated with cancer tissue specimens at a certain condition. Fluorescent microscope was used to observe fluorescent intensity of specimens. The fluorescent intensity were graded into 3 levels which included positive, doubtful and negative. The expression of cancer biological index was observed by the method of immunohistochemistry, the staining of which were also graded into 3 levels which contained positive, doubtful and negative. Kappa test or Spearman test were used to analyze the above correlation. RESULTS Only a fraction of breast cancer tissue specimen displayed positive fluorescent staining caused by FITC-LDP conjugate. 2 out of 3 iced specimens showed positive fluorescent staining. 11, 4 or 5 out of 20 wax specimens showed positive, doubtful and negative fluorescent staining, respectively. The fluorescent staining and ER, P53 or Bcl-2 immunohistochemistry staining had respective concordance with Kappa test ( P<0.05 ). The fluorescent staining and ER, P53 or Bcl-2 immunohistochemistry staining showed negative rank correlation, positive rank correlation and no rank correlation respectively with Spearman test(P<0.05). CONCLUSION LDP may have binding action on breast cancer cells, which might exist negative or positive correlation with ER or mutant P53 protein of breast cancer, respectively.
WNG Jin-kng;MENG Ru-song;LI Zhong-dong.
Study on Binding of Apoprotein of Lidamycin to Cancer Cells [J]. Chinese Pharmaceutical Journal, 2008, 43(16): 1228-1233
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