目的建立同时测定菝葜药材中落新妇苷和黄杞苷含量的HPLC方法。方法采用Agilent Extend-C₁₈(4.6 mm×250 mm,5μm)柱,以甲醇-水-磷酸溶液(35:65:0.1)为流动相,流速1.0 mL·min^-1,柱温35℃,检测波长为290nm。结果落新妇苷和黄杞苷分别在2.16×10^-3～1.08,3.176×10^-3～1.588μg内呈良好的线性关系;精密度试验RSD分别为0.75%,0.24%;重复性试验RSD分别为0.79%,0.89%;平均加样回收率分别为100.90%,98.71%。结论建立的方法可用于菝葜药材的质量控制。

OBJECTIVE To establish a HPLC method for the determination of astilbin and engeletin in Smilax china.METHODS The samples were separated at 35℃using a Agilent Extend-C₁₈column with methanol-water-phosphoric acid (35:65:0.1)as mobile phase.Flow rate was at 1.0 mL·min^-1and the detection wavelength was at 290 nm.RESULTS The calibration curves of astilbin and engeletin were linear over the ranges of 2.16×10^-3-1.08 and 3.176×10^-3-1.588μg,RSDS of the pricision test were 0.75% and 0.24%,RSDS of the reproducibility were 0.79% and 0.89% ,the average recoveries were 100.90 % and 98.71%,respectively.CONCLUSION The method can be used as a quality control method for Smilax china.