摘要
目的制备单抗CD33导向的三氧化二砷免疫蛋白毫微球(CD33-As2O3-BSA-NP)并检测其特异性结合APL原代细胞的活性。方法通过N-羟基琥珀酰亚胺基-3-(2-吡基二硫)-丙酸酯(SPDP)交联的方法制备免疫蛋白毫微球。玻片凝集实验、免疫荧光法、光镜和电镜、CD33-As2O3-BSA-NP结合的CD33数量测定、检测As2O3免疫毫微球的共价连接与活性。结果CD33-As2O3-BSA-NP的玻片凝集反应,免疫荧光染色均为阳性;光镜下可看见细胞周围结合微球,电镜下可看见细胞伸出伪足紧密地与免疫毫微球结合在一起;每1g CD33-As2O3-BSA-NP表面偶联的CD33抗体数量是14.5 mg。结论制备的CD33-As2O3-BSA-NP由共价键连接且特异性的结合APL原代细胞。
Abstract
OBJECTIVE To prepare arsenic trioxide-loaded albumin immuno-nanoparticles(As2O3-BSA-NP) targeted with Monoclonal Antibody CD33 and test its specific killing effect on APL original cells.METHODS Immuno-nanoparticles were prepared by methods of SPDP crosslinking.Immuno-nanoparticles and its activity were tested by slide agglutination test,immunofluorescent assay,microscopy and scanning electron microscopy,the number of CD33 junctioned the surface of As2O3-BSA-NP.RESULTS CD33-As2O3-BSA-NP was positive in slide agglutination test and immunofluorescent assay.Under microscopy,the APL original cel1s were rounded with immuno-nanoparticles.The scanning electronmicroscopy revealed the albumin immuno-nanoparticles were tightly connected with the APL original cells.The amount of CD33 juncted with the surface of one gram CD33-As2O3-BSA-NP were 14.5 mg.CONCLUSION CD33-As2O3-BSA-NP might specifically bind against APL original cel1s by covalent bond.
关键词
三氧化二砷 /
免疫毫微球 /
制备
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Key words
Arsenic trioxide /
immuno-nanoparticles /
preparation
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杨志文;杨木华;夏侯国论;李青松;朱亮.
三氧化二砷免疫毫微球的制备及鉴定[J]. 中国药学杂志, 2008, 43(02): 115-118
YNG Zhi-wen;YNG Mu-hu;XIHOU Guo-lun;LI Qing-song;ZHU Ling .
Study on Preparation and Determination of Arsenic Trioxide-Loaded Albumin Immuno-nanoparticles [J]. Chinese Pharmaceutical Journal, 2008, 43(02): 115-118
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脚注
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基金
江西省科技厅重大专项(E031101)
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