摘要
目的探讨葛根素(puerarin,Pur)的减肥作用。方法建立膳食诱导的营养性肥胖大鼠模型,对肥胖大鼠灌胃给药Pur混悬液6周,观察Pur干预前后体重、肥胖指数、睾肾周围脂肪质量、脂肪系数、脂肪细胞数量、脂肪细胞大小、血脂、血糖及胰岛素(INS)水平变化。结果给予Pur干预后,营养性肥胖大鼠体重、肥胖指数(Lee′s)、睾肾周围脂肪质量、脂肪系数及脂肪细胞大小均明显低于肥胖模型组(P<0.05或P<0.01);高、低剂量组血清甘油三酯(TG)、总胆固醇(TC)含量显著下降(P<0.05或P<0.01)。结论Pur对营养性肥胖大鼠有明显的减肥及改善脂质代谢的作用。
Abstract
OBJECTIVE To investigate the weight-reducing effect of puerarin on obese rats.METHODS After the obese model was built successfully,puerarin was administered intragastrically to obese rats for six weeks.Then,body weight,obese indexes(Lee′s),fat weight around kidneys and testis,fat index(the ratio of fat weight to body weight),adipocyte number,adipocyte size,blood lipid and serum glucose were detected.RESULTS Body weight,obese index,weight of fat tissue,fat index,adipocyte size in the high and low dose of puerarin groups were significantly lower than those in obese model group(P<0.05 or P<0.01).Meanwhile,the levels of serum cholesterol and triglyceride were decreased significantly(P<0.05 or P<0.01).CONCLUSION Administration of puerarin to rats for six weeks shows weight-reducing effect and improved lipid metabolism.
关键词
葛根素 /
营养性肥胖大鼠 /
减肥作用
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Key words
puerarin /
diet-induced obese rats /
anti-obesity
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张玉武;李新平;申付文;史群芳;朱爱思;黄璐.
葛根素对营养性肥胖大鼠的减肥作用及机制[J]. 中国药学杂志, 2008, 43(02): 110-114
ZHNG Yu-wu;LI Xing-ping;SHENG Fu-wen;SHI Qun-fng;ZHU i-si;HUNG Lu.
Anti-obesity Effect of Puerarin on Diet-induced Obese Rats [J]. Chinese Pharmaceutical Journal, 2008, 43(02): 110-114
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参考文献
[1] GRUNDY S M，BREWER H B，CLEEMAN J I，et al. Definition of metabolic syndrome：Report of the national heart，lung and blood institute/American Heart Association Conference on scientific issues retated to definition [J] . Circulation，2004，109（3）：433-438.
[2] JIN W S，PAN C Y. Global agreement on definition of metabolic syndrome of International Diabetes Federation [J] . Chin J of Endocrinol Metab（中华内分泌代谢杂志），2005，21(4)：1-2.
[3] QIAN B C. Making principle and method for obese animal model [J] . Chin Pharm Bull（中国药理学通报），1993，9（1）：75-76.
[4] LIU Q C，LIU X Y，DU J Z，et al. Experimental study on the obese modelinducing by diet at a short term [J] . Med J CAPF（武警医学），2004，15(5)：371-372.
[5] WANG J F，GUO Y X，NIU J Z，et al. Effects of Radix Puerariae flavones on liver lipid metabolism in ovariectomized rats [J] . World J Gastroenterol，2004，10(13)：1967-1970.
[6] WANG D Q，TIAN Y P. Effect of Purrarin-arginine composition on lipid metabolism in rats [J] . Chin J Clin Rehabilit(中国临床康复)，2005，9(15)： 220-222.
[7] FLIER J S，SCHERER P E. Adipose tissue: from lipid storage compartment to endocrine organ[J] .Diabetes，2005，54 (suppl1): 6-7.
[8] ZHANG Y，BI H M，GAN P Z. Effect of puerarin injection on the expression of protein kinase B in experimental insulin resistance rats [J] .Chin Pharm Bull（中国药理学通报），2004，20(3)：307-310.
[9] LI J J，BI H M. Effects of puerarin on GLUT4 in adipocyte of rats with insulin resistance [J] . Chin J Clin Pharmacol Ther（中国临床药理学与治疗学），2004，9(8)：885-888.
[10] MAO C P，GU Z L. Experimental effect of puerarin on the formation of advanced glycation end products and expression of RAGE in the arota of diabetic rats [J] .Chin Pharm Bull（中国药理学通报），2004，20(4)：393-397.
[11] MAO C P，GU Z L，CAO L，et al. Effect of the serum ICAM-1、ox-LDL and plasma TNF-α of puerarin on the diabetes rats [J] . Chin Tradit Herb Drugs（中草药），2005，36(1)：86-88.
[12] XU M E，XIAO S Z，SUN Y H，et al. The study of anti-metabolic syndrome effect of puerarin in vitro [J] . Life Sci（生命科学），2005，77（25）： 3183-3196.
[13] OKUNO A，TAMERMOTO H，TOBE K，et al. Troglitazone increases the number of small adipocytes without the change of white adipose tissue mass in obese zucker rats [J] .J Clin Invest，1998，101（6）：1354-1361.
[14] RIEUSSET J，CHAMBRIER C，BOUZAKRI K，et al. The expression of the p8-5alpha subunit of phosphatidylinositol 32kinase is induced by activation of the peroxisome proliferators-activated receptor gamma in human adipocytes [J] . Diabetologia，2001，44(5)：544-554.
[15] ZHENG X X，HU Y H，LIU J H，et al. Screening of active compounds as neuromedin U2 receptor agonist from natural products [J] . Bioorg Med Chem Lett，2005，15 ：4531-4535.
[16] LI X P，SHENG F W，ZHANG Y W，et al. A functional characterization of high throughput screening cell lines for human neuromedin U receptor subtype two specific agonists using a luciferase reporter gene assay. April25-27，2005[C] . Guangxi：Guilin.
[17] ZHENG X X，OUYANG K Q，HU Y H，et al. Progress on neuromedin U and its receptors [J] . Chin Pharm Bull（中国药理学通报），2004，20 (4)：373-377.
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脚注
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基金
国家自然科学基金资助项目(30471250);苏州大学医学发展基金资助项目(EE120612)
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