OBJECTIVE To investigate the effects of hydroxyethylpuerarin on expression of ICAM-1 and VCAM-1 in rats with focal cerebral ischemia-reperfusion injury and explore its mechanism of action.METHODS Rats were subjected to cerebral ischemia-reperfusion injury by middle cerebral artery occlusion(MCAO).After 2 h ischemia and 24 h reperfusion,the protein and mRNA expression of ICAM-1 and VCAM-1 were evaluated by Western blot and RT-PCR analysis.RESULTS Hydroxyethylpuerarin significantly reduced the expression of ICAM-1 and VCAM-1 in ischemic brain tissue after cerebral ischemia-reperfusion.CONCLUSION Hydroxyethylpuerarin may attenuate ischemic brain injury by down-regulation the expression of adhesion molecules in ischemic brain tissue.
LOU Hi-yn;WEI Xin-ing;WNG Ru-xi;SUN Xi;WNG Zi-ying;ZHONG Ying;ZUO Chun-xu;ZHNG Xiu-mei.
Effect of Hydroxyethylpuerarin on Expression of ICAM-1 and VCAM-1 in Rats with Focal Cerebral Ischemia-Reperfusion Injury [J]. Chinese Pharmaceutical Journal, 2008, 43(02): 104-107
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