OBJECTIVE To study the skin permeation of Huperzine A (Hup-A) in vitro transdermal patches in mouse,dog and human skins,and provide data for the formulation screening,pharmacodynamic and pharmacokinetics studies.METHODS In vitro permeation studies were conducted using two-chambered Valia-Chien glass diffusion cell assembly in three different skin species. Hup-A concentrations were determined by HPLC method,and permeation rates of Hup-A were calculated and compared.RESULTS The penetration of Hup-A through mouse skin was quicker and permeation rate was higher than that through dog and human skins. The permeation rate-time profiles of Hup-A through dog and human skins were similar.Hup-A permeation rate reached the maximum approximately 12～24 h following the application of the patch,and then maintained for up to 84 h.CONCLUSION The Hup-A permeation rates of mouse and human skins were greatly different. So,the mouse-based pharmacodynamics should be designed according to the in vitro Hup-A skin permeation study. In addition,the similarity of Hup-A skin permeation between dog and human skin supports the use of the dog as a species for evaluating the pharmacokinetics of transdermally-administered Hup-A.
YE Jin-cui;CHEN Guo-shen.
Skin Permeation of Huperzine A in Different Skin Species [J]. Chinese Pharmaceutical Journal, 2007, 42(14): 1095-1097
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