摘要
目的研究西替利嗪对P物质诱导表皮角质形成细胞系HaCaT细胞和真皮成纤维细胞表达单核细胞趋化蛋白-1(MCP-1)的影响。方法采用MTT法考察西替利嗪对两种皮肤细胞生存性的影响;ELISA方法测定P物质诱导两种皮肤细胞分泌MCP-1的时效关系与量效关系。以P物质刺激HaCaT细胞和真皮成纤维细胞,加入不同剂量的西替利嗪共孵育24h,测定西替利嗪对MCP-1表达的影响。结果以10-8mol·L-1P物质刺激HaCaT细胞24h可以显著增加HaCaT细胞和真皮成纤维细胞MCP-1的分泌量,不同浓度的西替利嗪均可以抑制皮肤细胞MCP-1的分泌。结论西替利嗪可能通过抑制趋化因子MCP-1的表达而发挥抗皮肤过敏作用。
Abstract
OBJECTIVE To study the effect of cetirizine on the expression of moncyte chemoattractant protein-1(MCP-1) induced by substance P(SP)in keratinocytes line HaCaT cells and fibroblasts.METHODS The time-and dose-response relationships of MCP-1 release of HaCaT cells and fibrobalsts stimulated by SP were determinated by ELISA method. The effect of cetirizine on the release of MCP-1 in HaCaT cells and fibrobalsts was also measured by using ELISA kit.RESULTS The expression of MCP-1 in HaCaT cells and fibrobalsts stimulated by SP (10-8mol·L-1) for 24 h was significantly increased. Cetirizine significantly decreased the production of MCP-1 in HaCaT cells and fibroblasts stimulated by SP.CONCLUSION The role of cetirizine on the cutaneous allergy may be related to inhibiting the expression of MCP-1.
关键词
西替利嗪 /
P物质 /
HaCaT细胞 /
真皮成纤维细胞 /
皮肤过敏 /
单核细胞趋化蛋白-1
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Key words
cetirizine /
substance P /
HaCaT cells /
fibroblasts /
cutaneous allergy /
moncyte chemoattractant protein-1(MCP-1)
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刘继勇;胡晋红;朱全刚;孙华君;彭程.
西替利嗪对P物质诱导皮肤细胞单核细胞趋化蛋白-1表达的影响[J]. 中国药学杂志, 2007, 42(14): 1067-1070
LIU Ji-yong;HU Jin-hong;ZHU Qun-gng;SUN Hu-jun;PENG Cheng.
Effect of Cetirizine on Expression of MCP-1 Induced by Substance P in HaCaT Cells and FiBroblasts [J]. Chinese Pharmaceutical Journal, 2007, 42(14): 1067-1070
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参考文献
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脚注
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基金
军队医药卫生十五重点项目基金(01Z66);上海市科委重大专项基金(04DZ19846)
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