OBJECTIVE To research the phase separation ability of different PEG/(NH4)2SO4 system and extraction of aloe polysaccharides by aqueous two phase system.METHODS Aloe polysaccharides was extracted from aloe gel by the mean of PEG 6 000/(NH4)2SO4 aqueous two phase system.The contents of polysaccharides was determined by phenol-sulfuric acid method according to the standard of D-mannose,the structure of aloe polysaccharides was analyzed by IR,UV.RESUILTS PEG 6 000/(NH4)2SO4 aqueous two phase system was chosen for its best phase separation ability,more aloe polysaccharides was extracted by using PEG 6 000/(NH4)2SO4 aqueous two phase system(75.63%),compared to ethanol-precipitated(68.39%),which more organic solvent was used.CONCLUSION PEG 6 000/(NH4)2SO4 aqueous two phase system is a good method for extracting aloe polysaccharides.
XING Jin-min;LI Fen-fng;LING Yi-zeng;LIU Ti-ping.
Determination and Extraction of Aloe Polysaccharides by Polyethylene Glycol/(NH4)2SO4 Two Phase System [J]. Chinese Pharmaceutical Journal, 2007, 42(07): 541-543
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