1.College of Life Science，Jilin University，Changchun 130012，China；2.Department of Laboratory，China-Japan Union Hospital，JiLin University，Changchun 130012，China
OBJECTIVE To prepare antimicrobial peptide spray and to investigate the inhibition effect of the spray on bacteria in vitro. METHODS Orthogonal test was employed to optimize the prescription.Cylinder plate method was used to detect the effect on the following bacteria:Pseudomonas aeruginosa,Staphylocococus aureus, Escherichia coli and different species of resistant Gram-positive or resistant Gram-negative bacteria. RESULTS Antimicrobial peptide spray had significant effect on bacteria growth inhibition in vitro. CONCLUSION The spray can be prepared feasibly and stably,and have significant effect in vitro growth inhibition on bacteria.
TIN Xio-le;MENG Qing-fn;ZHU Ki;XU Xue-song;GO Hi-ying;TENG Li-rong.
Preparation of Antimicrobial Peptide Spray and the Inhibition Effect on Bacteria [J]. Chinese Pharmaceutical Journal, 2006, 41(21): 1635-1637
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参考文献
[1] LI J M，ZHOU K Y，ZHANG S Q，et al. Antibacterial peptide of innate immunity and evolution[J] .J Nanjing Nor Univ(Nat Sci)(南京师大学报，自然科学版)，1999，22：16-33.
[2] MASSCHALCK B，VAN HOUDT R，MICHIELS C W. High pressure increases bactericidal activity and spectrum of lactoferrin and nisin[J] .Int J Food Microbiol，2001，64(3)：325-332.
[3] HANCOCK R E W，LEHRER R. Cationic peptides： a new source of antibiotics[J] .Trends in Biotechnology，1998，16：82-88.
[4] LU J M.Transderml Drug Delivery New Dosage Form(经皮给药新剂型)[M] .Beijing： People′s Medical Publishing House，1997：134-178.
[5] LU B.New Dosage Forms of Drug and New Technology(药物新剂型与新技术)[M] .Beijing： People′s Medical Publishing House，2002：353-409.
[6] ZHANG L X.Technology of Biochemistry(生化试验方法和技术，第二版)[M] . Beijing： Higher Education Press，1997：116-124.
[7] ChP(2000)Vol Ⅱ(中国药典2000年版.二部)[S] .Appendix ⅪA.
[8] PANG Y H. Prediction Method of Drug Stability Study(药物稳定性预测方法)[M] .Beijing： People′s Medical Publishing House，1984.
