摘要
目的研究林蛙抗菌肽喷雾剂的制备方法及该制剂的体外抑菌活性。方法采用正交设计方法确定喷雾剂处方,用紫外分光光度法测定制剂中的林蛙抗菌肽含量,杯碟法进行林蛙抗菌肽喷雾剂对铜绿色假单胞菌、金黄色葡萄球菌和大肠埃氏菌及临床多重耐药菌的体外抑菌试验。结果林蛙抗菌肽喷雾剂对上述菌均有抑制作用。结论该制剂制备工艺可行,性质稳定,且抑菌效果良好。
Abstract
OBJECTIVE To prepare antimicrobial peptide spray and to investigate the inhibition effect of the spray on bacteria in vitro. METHODS Orthogonal test was employed to optimize the prescription.Cylinder plate method was used to detect the effect on the following bacteria:Pseudomonas aeruginosa,Staphylocococus aureus, Escherichia coli and different species of resistant Gram-positive or resistant Gram-negative bacteria. RESULTS Antimicrobial peptide spray had significant effect on bacteria growth inhibition in vitro. CONCLUSION The spray can be prepared feasibly and stably,and have significant effect in vitro growth inhibition on bacteria.
关键词
抗菌肽 /
喷雾剂 /
抑菌试验
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Key words
antimicrobial peptide /
spray /
resistant bacterium
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田晓乐;孟庆繁;朱凯;徐雪松;高海鹰;滕利荣.
林蛙抗菌肽喷雾剂的制备及体外抑菌活性研究[J]. 中国药学杂志, 2006, 41(21): 1635-1637
TIN Xio-le;MENG Qing-fn;ZHU Ki;XU Xue-song;GO Hi-ying;TENG Li-rong.
Preparation of Antimicrobial Peptide Spray and the Inhibition Effect on Bacteria [J]. Chinese Pharmaceutical Journal, 2006, 41(21): 1635-1637
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参考文献
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脚注
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基金
吉林省科技发展工业重大项目(20020503-2)
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