1.College of Food Science & Nutritional Engineering，China Agricultural University，Beijing 100083，China； 2.Shandong Institute of Commerce and Technology，Jinan 250013，China； 3.Working Station for Postdoctoral Scientific Research，Institute of Biopharmaceuticals of Shandong Province，Jinan 250108，China
OBJECTIVE To investigate the effects of carbohydrates on the stabilization of pancreatic kininogenase(PKase)under various conditions. METHODS According to the activity remaining of PKase during refrigeration, repeated freeze-thawing, dehydration and high-temperature storage, the effects of carbohydrates on the stabilization of PKase were determined. RESULTS The stabilization of PKase activity was conferred by trehalose, lactose, sucrose, hyaluronic acid(HA)and the combination of trehalose and HA during refrigeration, repeated freeze-thawing, dehydration and high-temperature storage respectively. CONCLUSION The stability of PKase activity can be enhanced by carbohydrates, among which the combination of trehalose and HA was the most effective.
ZHNG Yu-hu;LING Pei-xue;JI o-ping;ZHNG Tin-min.
Effects of Carbohydrates on the Stabilization of Pancreatic Kininogenase [J]. Chinese Pharmaceutical Journal, 2006, 41(17): 1347-1349
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