目的建立测定半枝莲药材中野黄芩苷、野黄芩素、木犀草素和芹菜素含量的HPLC分析方法。方法采用C18柱,测定野黄芩苷以甲醇-0.1%醋酸水溶液(40∶60)为流动相;测定野黄芩素、木犀草素和芹菜素以乙腈-0.1%醋酸水溶液为流动相,梯度洗脱(0→40min,乙腈15%→60%)。流速为1.0mL·min-1,检测波长为335nm。结果野黄芩苷、野黄芩素、木犀草素和芹菜素分别在0.0124～0.992μg,0.006675～0.6675μg,0.00484～0.484μg和0.006875～0.6875μg内呈良好的线性关系。所测10份半枝莲药材中,野黄芩苷、野黄芩素、木犀草素和芹菜素的含量范围分别为0.147%～0.592%,0.028%～0.296%,0.007%～0.084%和0.011%～0.217%。结论本方法简便可行,适用于半枝莲药材中4种黄酮类成分的含量测定。
Abstract
OBJECTIVE To establish HPLC methods for the determination of scutellarin, scutellarein, luteolin and apigenin in Herba scutellariae Barbata. METHODS C18 column was used with methanol-0.1% acetic acid solution(40∶60)as the mobile phase to determine scutellarin. A C18 column was used with acetonitrile -0.1% acetic acid solution as the mobile phase to determine scutellarein, luteolin and apigenin, and a linear gradient elution from 15% to 60% acetonitrile in 40 min was applied. The flow rate was 1.0 mL·min-1,and the detection wavelength was at 335 nm.RESULTS The ranges of calibration curve of scutellarin, scutellarein, luteolin and apigenin were 0.012 4～0.99 2 μg,0.006 675～0.667 5μg,0.00 484～0.484μg and 0.006 875～0.687 5μg, respectively. By using the established HPLC methods, 10 samples of Herba scutellariae Barbata were analyzed. The results showed that the contents of scutellarin, scutellarein, luteolin and apigenin were 0.147%～0.592%, 0.028%～0.296%, 0.007%～0.084% and 0.011%～0.217%, respectively. CONCLUSION A simple, effective and feasible HPLC method to determine the contents of four flavonoids is established for the quality control of Herba scutellariae Barbata.
关键词
半枝莲 /
野黄芩苷 /
野黄芩素 /
木犀草素 /
芹菜素 /
高效液相色谱法
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Key words
Herba scutellariae Barbata /
scutellarin /
scutellarein /
luteolin /
apigenin /
HPLC
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参考文献
[1] Ch.P 2005 Vol. Ⅰ(中国药典2005年版一部)[S]：2005：77-78.
[2] ZOU Z L, WU Q N. Review on chemical constituents and pharmacological effects of Herba Scutellariae Barbata[J]. Lishizhen Med Mater Med Res(时珍国医国药), 2005, 16(2)：149-150.
[3] JIANG X G, GU Z L. Chemical constituents and pharmacological effects of Scutellaria barbata[J]. Chin Wild Plant Res(中国野生植物资源)，2004, 23(1)：3-5.
[4] WANG W S, ZHOU Y W, YE Y H, et al. Studies on the flavonoids in herb from Scutellaria barbata[J]. China J Chin Mater Med(中国中药杂志)，2004, 29(10)：957-959.
[5] YU J, LEI J, YU H, et al. Chemical composition and antimicrobial activity of the essential oil of Scutellaria barbata[J]. Phytochemistry, 2004，65(7)：881-884.
[6] LEE T K, KIM D I, SONG Y L, et al. Differential inhibition of Scutellaria barbata on HCG-promoted proliferation of cultured uterine leiomyomal and myometrial smooth muscle cells[J]. Immunopharmacol Immunotoxicol, 2004, 26(3)：329-342.
[7] LEE T K, LEE D K, KIM D I, et al. Inhibitory effects of Scutellaria barbata on human uterine leiomyomal smooth muscle cell proliferation through cell cycle analysis[J]. Int Immunopharmacol, 2004, 4(3)：447-454.
[8] SATO Y, SUZAKI S, NISHHIKAWA T, et al. Phytochemical flavones isolated from Scutellaria barbata and antibacterial activity against methicillin-resistant Staphylococcus aureus[J]. J Ethnopharmacol, 2000, 72(3)：483-488.
[9] ZOU Z L, WU Q N. Determination of total flavonoids in different parts of Scutellaria barbata[J]. J GX Tradit Chin Med Univ(广西中医学院学报), 2004, 7(3)：72-73.
[10] LI S Z, PAN H F. HPLC determination of scutellarin in Herba scutellariae Barbata[J]. Chin Tradit Herb Drugs(中草药), 2003, 34(2)：183-184.
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