摘要
目的对刺梨多糖进行分离纯化及神经营养活性研究。方法采用DEAE-纤维素柱色谱及Sepharose CL-6B凝胶柱色谱对刺梨粗多糖进行分离纯化,得到RRTP-1～RRTP-8共8个多糖组分。凝胶柱色谱和聚丙烯酰胺凝胶电泳鉴定纯度。采用PC12细胞对各组分的神经营养活性进行筛选,并对纯化的单一多糖进行活性定量测定。结果RRTP-1和RRTP-3为相对分子质量分布均一多糖。PC12细胞实验结果表明,多个多糖组分能刺激PC12细胞产生神经纤维样突起。结论多数刺梨多糖组分具有神经营养活性。
Abstract
OBJECTIVE To study the isolation and neurotrophic activity of polysaccharides from Rosa roxburghii Tratt.METHODS RRTP-1～RRTP-8 were isolated by DEAE-cellulose and Sepharose CL-6B column chromatography from crude polysaccharide.Their homogeneity was determined by gel column chromatography and electrophoresis.Neurotrophic activity of RRTP-1～RRTP-8 was screened by PC12 cell assay.Quantitative neurotrophic assays to homogeneous polysaccharides were carried out.RESULTS RRTP-1 and RRTP-3 were homogeneous polysaccharides.The screening results showed that most of polysaccharide fractions induced the differentiation of PC12 cell.CONCLUSION The most of polysaccharide fractions from Rosa roxburghii Tratt have obvious neurotrophic activity.
关键词
刺梨 /
多糖 /
分离纯化 /
神经营养 /
神经生长因子
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Key words
Rosa roxburghii Tratt /
polysaccharide /
isolation /
neurotrophic activity
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杨娟;杨付梅;孙黔云.
刺梨多糖的分离纯化及其神经营养活性[J]. 中国药学杂志, 2006, 41(13): 980-982
YNG Jun;YNG Fu-mei;SUN Qin-yun.
Study on Isolation and Neurotrophic Activity of Polysaccharides from Rosa roxburghii[J]. Chinese Pharmaceutical Journal, 2006, 41(13): 980-982
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参考文献
[1] WANG W，XIA B N. Advances of Rosa roxburghii Tratt[J] .Chin Pharm J (中国药学杂志)，1996，31 (11)： 643-645.
[2] FANG J N，DING K. Preparation and application of floral polysacchride and its derivatives from Nerium indicum mill [P] . Chin Pat(中国专利). (CN99125746.4)，24 Dec 1999，China.
[3] YANG J，WU M C，ZHANG S H. Study on isolation，purification and characteristics of polysaccharide Le-Ⅲ from the fruitbody of Lentinus edodes[J] .J Huazhong Agri Univ (华中农业大学学报)，1999，18 (2)：188-190.
[4] BITTER T，MUIR H M. A modified uronic acid carbazole reaction[J] . Anal Biochem，1962，4： 330-334.
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脚注
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基金
贵州省优秀科技教育人才省长专项资金
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