摘要
目的建立同时测定血浆中卡托普利和氢氯噻嗪浓度的高效液相色谱法,并将该方法应用到健康受试者口服复方卡托普利片后卡托普利和氢氯噻嗪血药浓度测定。方法采用Diamonsil C18(4.6 mm×150 mm,5μm)色谱柱,流动相:A:pH 1.8醋酸水溶液,B:乙腈,0～5 min,A-B=90∶10,8 min后,A-B=60∶40;柱温30℃,流速1.2 mL·min-1,检测波长263 nm,磺胺嘧啶为内标。卡托普利经对溴苯乙酰基溴(p-BPB)衍生化后同时测定卡托普利和氢氯噻嗪血药浓度。结果在一定浓度范围内,被测药物(卡托普利和氢氯噻嗪)分别和内标的峰面积之比与浓度成良好的线性关系,提取回收率均高于70%,日内和日间精密度均在合格范围内。结论本试验建立的方法简便、快速、准确,适用于复方卡托普利片剂中卡托普利和氢氯噻嗪体内血药浓度测定。
Abstract
OBJECTIVE To determinate of captopril and hydrochlorothiazide by high performance liquid chromatography.METHODS The HPLC separation was carried out on a Diamonsil C18(4.6 mm×150 mm,5 μm) column,using a UV detector and the wavelength was 263 nm.Elution was carried out with acetate acid solution(solvent A,pH 1.8) and acetonitrile(solvent B) at a flowrate of 1.2 mL·min-1.Gradient HPLC was used with thesolvent ratio changed from 90∶10(for 5 min),to 60∶40(after 8 min) for solvent A∶B,respectively.The internal standard was sulfadiazine.RESULTS The peak area ratios of captopril or hydrochlorothiazide to internal standard were linear within a certain range.The extraction recoveries were more than 70%.The relative standard derivations of within-day and between-day were less than 15%.CONCLUSION The method is proved to be accurate,sensitive and reliable.It is suitable for the determination in human plasma of the new combination formulation of captopril and hydrochlorothiazide.
关键词
高效液相色谱法 /
血药浓度 /
卡托普利 /
氢氯噻嗪
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Key words
HPLC /
plasma concentration /
captopril /
hydrochlorothiazide
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黄滔敏;何忠;郑晓伟;绍路平;段更利.
高效液相色谱法同时测定卡托普利和氢氯噻嗪的血药浓度[J]. 中国药学杂志, 2006, 41(13): 1010-1012
HUNG To-min;HE Zhong;ZHENG Xio-wei;SHO Lu-ping;DUN Geng-li.
Determination of Captopril and Hydrochlorothiazide in Serum by High Performance Liquid Chromatography [J]. Chinese Pharmaceutical Journal, 2006, 41(13): 1010-1012
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参考文献
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脚注
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