OBJECTIVE To prepare the dextran-sperminee polycation as the nonviral gene carrier.METHODS The carrier was prepared by the chemical synthesis,using the dextran,sodium periodate,spermine and sodium borohydride as the reactants.The main physical properties of the synthesized gene carrier were characterized by the methods of FTIR spectrophotometer,transmission electron microscope and laser nanoparticle detecter.The transfection with the dextran-sperminee polycation was performed using the pCMV-GFP as the reporter gene,the Transffectine 2000 liposome as the positive controller,the SMMC-7721 as transfected cells.The transfecting result was analyzed by fluorescence microscope instrument.The yield of transfection(%transfection)was calculated by counting the fluorescent cells in a certain area of a particular well,and dividing the number of fluorescent cells by the number of total cells in the same field.RESULTS The dextran and spermine were linked by the covalent bond.The size of the carrier was 9.0 nm,the Zeta potential was +31.1 mV.The carrier had a higher transfection efficency to SMMC-7721 cells.The yield of transfection was 31.1%.CONCLUSION The chemical synthesis method mentioned above can be used to prepare the optimum dextran-sperminee polycation nanoparticle as nonviral gene carrier.
M Shou-dong;CHEN Jin-hi.
Study on Synthesis and Characterization of Nonviral Gene Carrier of Dextran-Spermine Polycation [J]. Chinese Pharmaceutical Journal, 2006, 41(13): 1005-1009
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