目的检测药用胶囊中合成着色剂含量。方法高效液相色谱梯度洗脱法,流动相A为乙腈,B为0.02 mol·L^-1乙酸铵,洗脱程序:0～5 min,A相浓度8%～18%,B相浓度92%～82%;5～12 min,A相浓度18%～74%,B相浓度82%～26%;12～15 min,A相浓度74%～74%,B相浓度26%～26%。流动相流速为1.0 mL·min^-1,紫外检测波长254 nm,柱温25℃,进样量:10μL。结果药用胶囊中的合成着色剂回收率达93.2%～98.5%,精密度RSD在0.8%～3.0%,浓度范围在5～60 mg·L^-1时有良好的线性关系。结论应用此方法可使合成着色剂得到很好分离,测定结果满意。

OBJECTIVE To develop a high performance liquid chromatography(HPLC) for isolating and measuring the content of synthetic colorants in officinal capsule.METHODS HPLC with gradient elution was used in the contents measurement of 6 different colors capsules.Mobile phase A was acetonitrile,B was 0.02 mol·L^-1 ammonium acetate.Elution program:0～5 min,8%～18% of phase A and 92%～82% of phase B;5～12 min,18%～74% of phase A and 82%～26% of phase B;12～15 min,74%～74% of phase A and 26%～26% of phase B. The elution speed of the mobile phase was 1.0 mL·min^-1.The wave length of UV detector was 254 nm.The temperature of the column was 25 ℃,sample size was 10 μL.RESULTS 93.2%～98.5% of synthetic colorants in the capsules were recovered,the peak concentrations were at the range of 5～60 mg·L^-1.Relative standard deviation was at the range of 0.8%～3.0%.CONCLUSION Different synthetic colorants are completely separated by the method.