摘要
目的研究了酵母提取物、水解酪蛋白、真菌诱导子、茉莉酸及稀土元素等对甘草愈伤组织中甘草黄酮类化合物合成的影响。方法在培养基中添加各种附加物,研究其对甘草黄酮类化合物合成的影响。结果 0.1％的酵母提取物可使培养物中黄酮类化合物的含量提高76.8％,甘草查尔酮含量比对照提高7.5倍。水解酪蛋白在0.05％时,培养物中黄酮类化合物含量达最大值,为对照的1．7倍,其中甘草查尔酮的含量在0.1％时最高,为对照的3．2倍。50 mL培养基中加入真菌诱导子2 mL,甘草培养物中黄酮类化合物的含量为149．58μg·g-1,比对照(85.26μg·g-1)增加75％。10.0μmol·L-1茉莉酸使黄酮类化合物的含量比对照提高了41％。低浓度的稀土元素Eu3+对黄酮类化合物的生物合成具有明显的促进作用,培养基中添加0.1 mg·L-1的稀土元素Eu3+最有利于黄酮类化合物的积累。此时,黄酮类化合物的含量为对照的2.7倍,其中甘草素的含量是对照的4倍。结论在甘草愈伤组织培养过程中,添加酵母提取物、水解酪蛋白、真菌诱导子、茉莉酸及稀土元素等是提高黄酮类化合物的有效手段之一。
Abstract
OBJECTIVE To investigate the effects of yeast extract, casein hydrolysate, fungal elicitor, JA and rare-earth element Eu3+ on flavonoids production in Glycyrrhiza uralensis callus.METHODS The flavonoids content in Glycurrhiza uralensis-1 was obtained with the addition of 2 mL fungal elicitor to the medium, which was 75% increased compared with the control. The JA concentration of 10.0 μmol·L-1 was found to be the most suitable for the flavonoids accumulation with 41 % higher than control. Flavonoids content was as 2.7 times as that of the control, and liquiriti-genin was as 4 times as that of the control when the concentration of rare-earth element Eu3+ was 0.1 mg·L-1.CONCLUSION The addition of yeast extract,casein hydrolysate, fungal elicitor, JA and rare-earth element Eu3+ to the medium are effective approaches to enhance flavonoids accumulation in G.uralensis calli.
关键词
甘草 /
愈伤组织 /
酵母提取物 /
水解酪蛋白 /
真菌诱导子 /
茉莉酸 /
稀土元素 /
黄酮类化合物
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Key words
Glycyrrhiza uralensis /
callus /
yeast extract /
casein hydrolysate /
fungal elicitor /
jasmonic acid /
rare-earth element /
flavonoids
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杨世海;刘晓峰;果德安;郑俊华.
不同附加物对甘草愈伤组织培养中黄酮类化合物形成的影响[J]. 中国药学杂志, 2006, 41(02): 96-99
YNG Shi-hi;LIU Xio-feng;GUO De-n;ZHENG Jun-hu.
Effects of Different Additives on Accumulation of Flavonoids in Glycyrrhiza uralensis Callus [J]. Chinese Pharmaceutical Journal, 2006, 41(02): 96-99
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