摘要
目的探讨塔斯品碱盐酸盐(TA)对成纤维细胞作用及促进伤口愈合的机制。方法体外培养L929成纤维细胞,经不同质量浓度塔斯品碱盐酸盐(0.05,0.1,0.2,0.4和0.5 μg·mL-1)作用后,以及加入0.4 μg·mL-1塔斯品碱盐酸盐作用不同时间(24,48和72 h)后,采用ELISA测定其自分泌转化生长因子-β1(TGF-β1)及表皮生长因子(EGF)的变化规律。结果塔斯品碱盐酸盐可促进成纤维细胞自分泌TGF-β1和EGF,且随质量浓度的增大,作用时间的延长而增强。结论塔斯品碱盐酸盐可促进TGF-β1和EGF的自分泌,促进成纤维细胞及炎性细胞的趋化性,促进细胞外基质的沉积,促进创面愈合。
Abstract
OBJECTIVE To investigate the mechanism of enhancing wound healing by taspine hydrochloride (TA) .METHODS Fi-broblasts were cultured in vitro, and TA was applied with different concentrations (0.05,0.1,0.2,0.4 and 0.5 μg·mL-1) and time (24,48 and 72 h) to influence their autocrine. The levels of transforming growth factor-β1 (TGF-β1) and epidermal growth factor (EGF) were determined by enzyme-linked immunoadsorbent assay (ELIAS) .RESULTS TA increased autocrine of TGF-β1 and EGF by fibroblasts,the action was reinforced with the increasing TA concentration and the extending of time.CONCLUSION The above results indicated that TA increased autocrining of TGF-β1 and EGF, and accelerated the chemotaxis of fibroblast and inflammation cell, the deposition of extracellular matrix and wound healing .
关键词
塔斯品碱盐酸盐 /
转化生长因子-β1 /
表皮生长因子 /
创面愈合
{{custom_keyword}} /
Key words
taspine hydrochloride /
transforming growth factor-β1
{{custom_keyword}} /
董亚琳;贺浪冲;陈方.
塔斯品碱对体外培养成纤维细胞自分泌生长因子的影响[J]. 中国药学杂志, 2005, 39(11): 826-828
DONG Y-lin;HE Lng-chong;CHEN Fng.
Effect of taspine hydrochloride on autocrining growth factor of fibroblast in vitro[J]. Chinese Pharmaceutical Journal, 2005, 39(11): 826-828
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
参考文献
[1］中国医学科学院药物研究所.中药志[M］.北京：人民卫生出版社，1979：424.
[2］Milanowski DJ，Winter RE，Elvin-Lewis MP. Geographic distribution of three alkaloid chemotypes of Croton lechleri[J］.J Nat Prod，2002，65(6)：814.
[3］Porras-Reyes BH， Lewis WH， Roman J. Enhancement of wound healing by the alkaloid taspine defining mechanism of action [J］. Proc Soc Exp Biol Med，1993，203(1)：18.
[4］贺浪冲，耿信笃.细胞膜受体色谱法——研究药物与受体作用的新方法[J］.生物医药色谱新进展，1996，3，8.
[5］He LC， Yang GD， Geng XD. Enzymamatic activity and chromatographic characteristics of the cell membrane immobilization silica surface[J］.Chin Sci Bull，1999，44(9)：826.
[6］王益民，韦福康，刘敏，等. 丹参对体外培养成纤维细胞自分泌生长因子的影响[J］.中国修复重建外科杂志，2002，6(16)：408.
[7］Lekic P，Mcculloch CA.Periodontal ligament cell populations： The centra role of fibroblasts in creating a unique tissue[J］.Anat Rec，1996，245(2)：327.
[8］Bhora FY， Dunkin BJ， Batzri S，et al. Effect of growth factors on cell proliferation and epithelialization in human skin[J］.J Surg Res， 1995，59(2)：236.
[9］Fu XB， Shen ZY， Chen YL，et al. Randomised placebo controlled trail of use of topical recombinant bovine basic fibroblast growth factor for second-degree burns [J］.Lancet， 1998， 352 (9141)：1661.
[10］付小兵，王正国. 现代高新技术与创伤修复[M］. 北京：人民军医出版社，2002：282～283.
[11］Vaisberg AJ，Milla M，Planas MC.Taspine is the cicatrizant principle in Sangre de Grado extracted from Croton lechleri[J］. Planta Med，1989，55(2)：140.
{{custom_fnGroup.title_cn}}
脚注
{{custom_fn.content}}
