目的观察海洋贝类提取物(EMS)对大鼠血浆及主动脉壁中血栓素B₂(TXB₂)、6-酮-前列腺素F_1α(6-Keto-PGF_1α)水平的影响以及EMS对脉动血栓形成时间的影响,以探讨EMS抗动脉粥样硬化(AS)的作用机制。方法将大鼠随机分组,药物均经灌胃给药。采用放射免疫法(RIA)测定各组大鼠血浆及主动脉壁中。TXB₂,6-Keto-PGF_1α的含量;采用电刺激损伤法测定大鼠颈动脉血栓形成时间,观察EMS的抗血栓作用。结果EMS5.4,10.8g·kg^-1(相当于鲜贝)可明显降低大鼠血管壁中TXB₂含量(P<0.05);10.8g·kg^-1可减少血浆中TXB₂水平(P<0.05);EMS对血管壁及血浆中6-Keto-PGF_1α无明显影响(P>0.05);EMS10.8g·kg^-1可延长大鼠颈动脉血栓形成时间(P<0.05)。结论EMS能降低血浆及血管壁中血栓素A₂水平,具有调节TXA₂与前列环素平衡、抑制血栓形成的作用。

OBJECTIVE To investigate the influence of the comprehensive extract from marine shellfish (EMS) on the content of throm-boxane B₂(TXR₂,metabolite of thromboxane A₂) , 6-Keto-PGF_1α in plasma and aortic wall. To study the effect on the time of arterial thrombosis and investigate the mechanism of anti-atherosclerotic action. METHODS The concentrations of TXB₂ and 6-Keto-PGF_1α in plasma and aortic wall were determined by radio immunity analyse(RIA) . The experimental carotid artery thromobosis in rat model was evaluated by electric stimulation injury. Then the activity of anti-thromobosis of EMS was investigated.RESULTS EMS(5.4,10.8 g·kg^-1) significiantly decreased the amount of TXB₂, in aortic wall (P< 0.05)and decreased the level of TXB₂ in plasma(P< 0.05). EMS(2.7, 5.4 and 10.8 g·kg^-1) didn't significantly effect on the concentration of 6-Keto-PGF_1α(P> 0.05).EMS(10.8 g·kg^-1) obviously prolonged the thromobosis time in rats(P<0.05).CONCLUSION The anti-athersclerotic mechanism of EMS may be related to the reduction of TXB₂in plasma and aortic wall and the inhibition of thrombosis.