摘要
目的建立大鼠尿液中白藜芦醇的含量测定方法。方法实验以聚酰胺固相萃取小柱对大鼠尿液进行净化处理,采用中心复合设计-响应曲面法对聚酰胺固相萃取条件进行优化,并将优化的实验条件应用于尿液中白藜芦醇的含量测定。结果10%,30%,100%甲醇洗脱液体积的优选值分别为10,5,4 mL。尿样中白藜芦醇在0.043～8.600mg·L-1内关系良好(r=0.999 8),最低定量限为0.043 mg·L-1;低、中、高3种浓度下的回收率、日内及日间精密度均符合方法学要求结论该法简便、专属性高,能很好地去除内源性物质的干扰,可用于尿液中白藜芦醇浓度测定。
Abstract
OBJECTIVE To develop a optimized method for the determination of trans-resveratrol in rat urine. METHODS The sample was processed by using solid-phase extraction with polyamide cartridges. A central composite design, which allowed a sequential response surface methodology to he performed, was used for the optimization of the extraction procedure. RESULTS The optimized condition for SPE was achieved by using 10% methanol 10 mL,30% methanol 5 mL and 100% methanol 4 mL. The linear range was obtained from 0.043 to 8.6 mg·L-1 and the limit of detection was 0,043 mg·L-1. The average recovery was 98.0% and the RSDs of intra-day and inter-day were less than 10%.CONCLUSION A reliable and specific SPE-HPLC method for the determination of resveratol in rat urine was developed. This method effectively decreased the interference of endogenous compounds, and was appropriate for the determination of resveratrol in rat urine.
关键词
响应曲面 /
中心复合设计 /
固相萃取 /
白藜芦醇
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Key words
response surface methodology /
central composite design /
solid-phase extraction /
resveratrol
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汪冬庚;刘文英.
测定大鼠尿液中白藜芦醇含量时响应曲面法在样本固相萃取条件优化中的应用[J]. 中国药学杂志, 2005, 39(09): 710-713
WNG Dong-geng;LIU Wen-ying.
Application of response surface methodology in the optimization of solid-phase extraction condition for the determination of trans-resveratrol in rat urine [J]. Chinese Pharmaceutical Journal, 2005, 39(09): 710-713
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脚注
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