摘要
目的建立液-质联用(HPLC-MS)测定人血浆中地塞米松浓度的方法。方法采用Agilent Zorbax Extend C18色谱柱(2.1mm×150 mm,5μm),以0.4%醋酸铵溶液-乙腈(62:38)为流动相,流速0.25 mL·min-1,在血浆中加入内标醋酸泼尼松,固相萃取后采用HPLC-MS(ESI源)测定地塞米松的血浆浓度。结果地塞米松在人血浆中0.02～4 mg·L-1内线性良好,最低检测限为0.02 mg·L-1,绝对回收率>95%,相对回收率在98.84%～102.80%之间,日内及日间RSD均小于3%。结论本方法能满足人血浆中地塞米松浓度的测定。
Abstract
OBJECTIVE To develop and validate a HPLC-MS method for determining dexamethasone in human plasma.METHODS The separation was performed on a column of Zorbax Extend C18(2.1mm×150 mm,5 μm). The mobile phase consisted of 0.4% ammonium acetate solution-acetonitrile(62-38) .Using solid phase extraction, dexamethasone in human plasma was measured by HPLC-MS with ESI. The internal standard was prednisone acetate.RESULTS The standard curve was linear over the range of 0.02～4 mg·L-1. The lowest dexamethasone concentration that gave a reproducible integration was 0.02 mg·L-1.The absolute recovery was more than 95%,the relative recovery was among the range of 98.84%～102.80%.The RSDs of intra-day and inter-day validation were less than 3% . CONCLUSION HPLC-MS may be used for the determination of dexamethasone in human plasma.
关键词
地塞米松 /
高效液相色谱-质谱 /
血药浓度
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Key words
dexamethasone /
HPLC-MS /
concentration
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刘蕾;付逸龙;殷琦;李可欣;孙春华.
SPE-HPLC-MS测定人血浆中地塞米松浓度[J]. 中国药学杂志, 2005, 39(09): 705-707
LIU Lei;FU Yi-long;YIN Qi;LI Ke-xin;SUN Chun-hu.
Determination of dexamethasone in human plasma by SPE-HPLC-MS [J]. Chinese Pharmaceutical Journal, 2005, 39(09): 705-707
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参考文献
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脚注
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