目的快速诱导藏红花球茎愈伤组织，解决藏红花资源短缺问题。方法系统地研究外植体来源、培养基、温度、光照和激素因素对藏红花球茎诱导愈伤组织的影响。结果以出芽后芽周围2 cm左右的球茎为外植体，MS为培养基，2.0 mg·L-1 2，4-D 和0.25 mg·L-1 6BA为激素，在(21±0.3)℃，黑暗环境下培养，可以在14 d以后获得60%的愈伤组织诱导率。结论外植体和激素的选择是快速诱导藏红花愈伤组织的关键。
Abstract
OBJECTIVE To rapidly induce Crocus sativus callus taking corm as explant.〖WT5”HZ〗METHOD S The effects of corm source,various medium,temperature,light,and plant hormones on the inducing Crocus sativus callus were systematically and extensively investgated.〖WT5”HZ〗RESULTS The Crocus sativus corm callus was rapidly reduced within 14 days at (21±0.3)℃,in darkness by taking the corm of 2 cm diameter around shoot as explant,MS as medium,2.0 mg·L-1 2，4-D 0.25 mg·L-1 6-BA as plant hormones and the inducing rate reached 60%.〖WT5”HZ〗CONCLUSION The explant and the plant hormones were the key factors for callus induction.
关键词
藏红花 /
球茎 /
愈伤组织 /
快速诱导
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Key words
Crocus sativus L /
corm /
callus /
rapid induction
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