目的 建立适合盐酸西替利嗪血浆浓度测定的反相高效液相色谱法(RP-HPLC)。方法 选择离子对反相高效液相色谱法，以乙腈-0.02 mol·L-1磷酸二氢钠-三乙胺(50∶50∶0.15，内含十二烷基磺酸钠0.007 mol·L-1)为流动相，在波长229 nm处检测。结果 在10～400 ng·ml-1的浓度范围内血浆中西替利嗪具有良好的线性关系，平均萃取回收率大于70%，日内日间精密度均小于5%。结论 本法适用于测定血浆中西替利嗪的浓度，并进一步用于研究西替利嗪在人体内药代动力学过程。
Abstract
OBJECTIVE To establish a RP-HPLC method for the determination of cetirizine in plasma. METHODS Cetirizine was determined by a reversed-phase ion-paired high-performance liquid chromatography with the detection wavelength at 229 nm.The mobile phase was ACN:0.02 mol·L -1phophate:TEA 50∶50∶0.15)，containing 0.007 mol·L-1SDS. RESULTS The calibration curve was linear within the concentration range of 10～400 ng·ml-1of cetirizine in plasma.The mean extraction recovery was more than 70% and the relative standard deviations for the within-day and the between-days variation were all less than 5%. CONCLUSION This new method could be used to determine cetirizine in plasma and will be suitable to the pharmacokinetic study of cetirizine.
关键词
西替利嗪 /
反相高效液相色谱法 /
血药浓度
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Key words
cetirizine /
RP-HPLC /
plasma concentration
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参考文献
［1］Seidel WF,Cohen S,Bliwisc NG,et al.Cetirizine effects on objective measures of daytime sleepiness and performance［J］.Ann Allergy,1987,59:58.
［2］Pechadre JC,Vernay D,Tro.lese JF,et al.Comparison of the central and peripheral effects of cetirizine and terfenadine［J］.Eur J Clin Pharmacol,1988,35:255.
［3］Gengo FM,Dabronzo J,Yurchak A,et al.The relative antihistaminic and psychomotor effects of hydroxyzine and cetirzine［J］.Clin Phar-macol Ther,1987,42:265.
［4］Grant GA,Glovsky MM,Meltzer EO,et al.Safety and efficacy of ceti-rizine (CET) in the prophylactic management of patients with seasonal allergic rhinitis (SAR) and asthma,Abstract 225［J］.J Allergy Clin Immunol,1993,91:197.
［5］Chaweewan B,Jareonchai P,Dhorranintra B.A clinical comparison of cetirizine versus astemizole in perennial allergic rhinitis［J］.Drug in-vestigation,1993,5:222.
［6］Goh Cl,Wong WK,Lim J.Cetirzine vs placebo in chronic idiopathic urticariaa double blind randomised crossover study［J］.Ann Acad Med,1991,20:328.
［7］Hannuksela M,Kakimo K,Lammintausta K,et al.Dose ranging study:cetirizine in the treatment of atopic dermatitis in adults［J］.Ann Allergy,1993,70:127.
［8］Lefebvre RA,Rosseel MT,Bernheim J.Single dose pharmacokinetics of cetirizine in young and elderly volunteers［J］.Int J Clin Pharm Res,1988;8(6):463.
［9］Freiser HH,Newlan MP,Gooding DL,et al.Reversed phase high per-formance liquid chromatography of basic drugs on a silanol deactivated supported［J］.J Liq Chromatogr,1989,12(5):827.
［10］Kiel JS,Morgan SL.Computer-assisted optimazation of a high perfor-mance liquid chromatographic separation for chlorpromazine and thir-teen metabolites［J］.J Chromatogr,1989,485(1):827.
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