目的 建立反相高效液相色谱-紫外法测定血浆中沙丁胺醇浓度的方法。方法采用Ullraspher Cyano分析柱(250 mmx 4.6mm，5 μm),乙腈-甲醇-0.025 mol.I/1磷酸铵缓冲液(pH2.8)(2:0.5:97.5)为流动相,吗啡作内标.样品经离子对萃取纯化后进样,在224 nm波长下检测,按内标法定量.结果标准曲线在0.5~32μg.L1内有良好线性,最低检测浓度为0.3 μg-L,萃取回收率在93% -99%之间,方法回收率在98%～109%之间，日内RSD小于5%，日间RSD小于8%。结论本法具有快速简便,灵敏准确等特点，已用于测定沙丁胺醇缓释制剂生物利用度样品700余个,结果良好.

OBJECTIVE This paper established a high-performance liquid chromatography for the determination of salbutamol (SBA) in human plasma using morphine as the internal standard. METHOD A variable wavelength UV detector and Ultraspher Cyano column (5μm, 250 mmx4.6 mm,Beckman)were used.To the plasma sample 1.0,0.5 ml phosphate buffer (2.0 mol·L-1 ,pH9.0) containing0.4% diphe-nylboric acid 2-aminoethyl ester was added, then extracted with 4.0 ml chloroform containing 1% tetraoctylammonium bromide. The organic layer was removed and extracted again with 200μl (0.08 mol·L-1) acetic acid. 100 of the acid layer was injected onto the column .The mobile phase of acetonitrile-methanol-pH2.8,0.025 mol? L_1 phosphate buffer (2:0.5:97.5) was. pumped at the rate of 1.0 ml?min-1 through the column. The detector at 0.002 AUFS was set at 224 nm. RESULTS The retention times for SBA and morphine were 7.0 min and 8.3 min,respectively.The standard curve was linear over the concentration range of 0.5 to 32 μg·L-1 .The detection limit in plasma was 0.3μg·L-1.Extraction recovery was 93% ~ 99% ；The method recovery was 98% ~ 109% ；with intra-day RSD less than 5% ；inter-day RSD less than 8% . CONCLUSION This method was found to be simple,rapid,sensitive and accurate for the determination of SBA in human plasma.