目的：建立麦角菌发酵产物a麦角隐亭的RP-HPLC定量测定方法，对在一定条件下a麦角隐亭发酵生产的峰值进行初步探索。方法：色谱柱(250 mm×4.6 mm，填料：YWG-C，10μm)；流动相为甲醇-磷酸盐缓冲液(pH7.0)-乙酸乙酯(75：25：1)；流速为1.0 ml·min；检测波长为312 nm。建立回归方程，进行方法精密度测定和加样回收率试验。结果：回归方程A=19058.54M+1352.66，r=0.9997，在0.6375～6.4375μg范围内线性关系良好；日内和日间相对标准偏差(RSD)分别为1.15%和0.44%；标准品浓度为122，244和488μg·ml时加样回收率分别为108.48%，105.15%，109.53%。用该方法测得a麦角隐亭发酵生产的峰值一般在发酵的第15～17d。结论：该方法在定量测定麦角菌发酵产物a麦角隐亭方面灵敏、准确。

OBJECTIVE: To develop a reversed phase HPLC method for the quantitative determination of the ergot fermentation product α-ergocryptine and to determine the peak value of α-ergocryptine yielded during the fermentation. METHODS: A YWG-C₁₈column (250 mm×4.6 mm ID,particle size,10 μm) was used;the mobile phase was MeOHphosphate buffer (pH7.0)EtOAc (75∶25∶1) with a flow rate of 1.0 ml·min^-1and detected at 312 nm.A regression equation was set up,the precision of the method and the recovery rates were measured. RESULTS: Regression equation was A=19058.54M+1352.66,r=0.9997,over the range of 0.6375～6.4375 μg;the RSD for the intra day and inter day was 1.15%,0.44%,respectively;the mean recoveries were 108.48%,105.15% and 109.53% versus the added authentic sample at the concentrations of 122,244 and 488 μg·ml^-1,respectively.The peak value appeared between day 15 to day 17 under the given condition. CONCLUSION: The method is sensitive and reproducible for the quantitative determination of the ergot fermentation product α-ergocryptine.