天竺葵花抗氧化作用的研究

许申鸿

中国药学杂志 ›› 2000, Vol. 35 ›› Issue (04) : 234-236.

中国药学杂志 ›› 2000, Vol. 35 ›› Issue (04) : 234-236.
中药及天然药物

天竺葵花抗氧化作用的研究

  • 许申鸿
作者信息 +

Study on antioxidative effect of Pelargonium hortorum Bailey

  • Xu Shenhong
Author information +
文章历史 +

摘要

目的:研究天竺葵花的抗氧化作用。方法:用化学发光法研究天竺葵花粗提液对超氧阴离子自由基(O〈sub〉2〈/sub〉-)和羟在自由基(·OH)的清除作用。用分光光度法研究它对1,1一二苯基-2-苦肼基自由基(DPPH·)的清除作用。结果:天竺葵花具有很强的清除自由基活性。其粗提液清除O〈sub〉2〈/sub〉-的IC〈sub〉50〈/sub〉分别为3.5μg·ml〈sup〉-1〈/sup〉(红花),3.0μg·ml〈sup〉-1〈/sup〉(粉花),1.6μg·ml〈sup〉-1〈/sup〉(浅粉花);清除·OH 的IC〈sub〉50〈/sub〉分别为5.7μg·ml〈sup〉-1〈/sup〉(红花),4.2μg·ml〈sup〉-1〈/sup〉(粉花),3.8μg·ml〈sup〉-1〈/sup〉(浅粉花);清除DPPH.的IC〈sub〉50〈/sub〉分别为12μg·ml〈sup〉-1〈/sup〉(红花),8.3μg·ml〈sup〉-1〈/sup〉(粉花),5.7μg·ml〈sup〉-1〈/sup〉(浅粉花),均与市售茶多酚的清除能力相近(其IC〈sub〉50〈/sub〉。分别为:1.1,4.1,5.4μg·ml〈sup〉-1〈/sup〉)。结论:天竺葵花可能成为一种新的高效天然杭氧化剂。

Abstract

OBJECTIVE: To study the antioxidative effects of Pelargonium hortorum Bailey flower water extracts. METHODS: Scavenging effects of Pelargonium hortorum Bailey flower extracts on superoxide radical (O 2)and hydroxyl radical (·OH) was studied by a chemiluminescence assay,and the scavenging effect of them on 1,1diphenyl 2 picrylhydrazyl (DPPH·) was studied by spectrophotometry. RESULTS: The scavenging activity of Pelargonium hortorum Bailey flower extracts on free radical was strong.Its IC50on O2was 3.5 μg·ml-1(red),3.0 μg·ml-1(pink),1.6 μg·ml-1(lihgt pink),its IC50on ·OH was 5.7 μg·ml-1(red),4.2 μg·ml-1(pink),3.8 μg·ml-1(light pink),its IC50on DPPH· was 12 μg·ml-1(red),8.3 μg·ml-1(pink),5.7 μg·ml-1(light pink),similar to that of tea polyphenol(IC50=1.1,4.1,5.4 μg·ml-1). CONCLUSION: The Pelargonium hortorum Bailey flower can be used as an antioxidant.

关键词

天竺葵花 / 清除作用

Key words

Pelargonium hortorum Bailey flower / scavenging effect / free radical

引用本文

导出引用
许申鸿. 天竺葵花抗氧化作用的研究[J]. 中国药学杂志, 2000, 35(04): 234-236
Xu Shenhong . Study on antioxidative effect of Pelargonium hortorum Bailey[J]. Chinese Pharmaceutical Journal, 2000, 35(04): 234-236

参考文献

[1]王云章.庭院花卉的药用.太原:山西科学教育出版社.1986:84. [2]许申鸿,杭瑚.邻苯三酚-碳酸盐缓冲液化学发光体系的研究生物化学与生物物理进展,1999,26(5):488. [3]范小兵,李慈娟,沙大年,等.邻菲罗琳化学发光体系测定经自由基的建立.基础医学与临床,1998,18(6):68. [4]Mellors A,Tappel A.L.The inhibition of mitochondrial peroxi-dation by ubiquinone and ubiquinol.J Biol Chem,1996,241(19):4353. [5]Cotelle N,Bemier JL,Catteau JP et al.Antioxidant properties of hydroxy-flayvones.Fre Rad Biol Med,1998,20(1):35. [6]Cavin A,Hostettmann K,Dyatmyko W,et al.Antioxidant and Lipophilic Constituents of Tinospora crispa.Planta Mert,1998,64:393. [7]Sebastian SL,Ramps E,Ibanez E,et al.Dearomatization of An-tioxidant Rosemary Extracts by Treatment with Supercritical Carbon Dioxide.J Agric Faod Chem,1998,46:13 [8]Cheng Z,Kuo SC,Chan SC,et al.Antioxidant properties of butein isolated from Ihlbergia odorifera. Biochimica et Biophys-ica Acta,1998,1392:291. [9]Bocco A,Cuvelier ME,Richard H,et al.The antioxidant activity of various phenolic compounds measured by an accelerated test based on citronellal oxidation.Sciences Des Aliments,1998,18:13

48

Accesses

0

Citation

Detail

段落导航
相关文章

/