摘要
目的:观察丁基苯酞 (3-n-butylphthalide,NBP)对低糖低氧刺激后培养的胎鼠皮层神经细胞谷氨酸和5-HT释放的影响?方法:胎鼠皮层神经元以低糖低氧培养基培养造成低糖低氧模型;谷氨酸和5-HT以荧光分光光度法进行测定?结果:低糖低氧10h可使神经细胞谷氨酸的释放量增加,dl NBP(1和10μmol·L-1)和l-NBP(10μmol·L-1)可降低细胞培养基中谷氨酸的浓度,而同剂量的d-NBP对谷氨酸的释放无明显影响?dl-NBP和l-NBP亦可抑制由花生四烯酸 (100μmol·L-1)引起的神经细胞谷氨酸的释放?本实验结果还显示,dl-NBP(1和10μmol·L-1)及l-NBP(10μmol0·L-1)可降低低糖低氧后神经细胞培养基中5-HT的浓度?结论:dl-NBP和l-NBP可抑制谷氨酸和5-HT的释放,这可能是其抗脑缺血作用的机理之一?
Abstract
OBJECTIVE:To investigate the effects of dl-3-n-butylphthalide (dl-NBP),d-NBP and l-NBP on the release of glutamate and 5-hydroxytryptamine (5-HT) from cultured perinatal cortical neurons.METHODS:The cortical neurons were cultured with medium containing low glucose and bubbled with N2,Glutamate and 5-HT were determined fluorometrically.RESULTS:The results showed that dl-NBP (1 and 10μmol·L-1) and l-NBP (10μmol·L-1) reduced the glutamate release and the release of 5-HT from cultured cortical neurons induced by hypoglycemia/hypoxia for 10 h.d-NBP was shown no significant effect.dl-NBP and l-NBP also decreased the glutamater elease induced by arachidonic acid (100μmol·L-1).CONCLUSION:The inhibitory effects of NBP on the release of glutamate and 5-HT may be one of its action mechanisms in the treatment of cerebral ischemia.
关键词
丁基苯酞 /
神经细胞 /
低糖低氧 /
谷氨酸 /
5-HT
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Key words
3-n-butylphthalide /
neuron /
hypoglycemia /
hypoxia /
glutamate /
5-HT
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种兆忠;冯亦璞.
丁基苯酞对大鼠皮层神经元损伤后谷氨酸和5-羟色胺释放的影响[J]. 中国药学杂志, 1999, 34(09): 589-591
Chong Zhozhong.
Effects of NBP on the release of glutamate and 5-HT from cultured neurons subjected to hypoglycemia/hypoxi[J]. Chinese Pharmaceutical Journal, 1999, 34(09): 589-591
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参考文献
1 Nedegaard P，Engberg I，Flatman JA．The modulation of excitatory amino acid response by serotonin in the cat neocortex in virto．Cell Mol Neurobiol，1987，7(4)：367．
2 Reynolds JN，Baskys A，Carlen PL．The effects of serotonin on Nmethl-D-aspartate and synaptically evoked depolarization in rat neocortical neurons．Brain Res，1988，456(2)：286．
3 Sharma HS，Olsson Y，Dey PK．Blood-brain barrier permeability and cerebral blood flow following elevation of circulating serotonin level in the anaesthetied rats．Brain Res，1990，517(1-2)：215．
4 Winkler T，Sharma HS，Stalberg E，et al．Impairment of blood brain barrier function by serontonin induces desynchronization of spontaneous cerebral cortical activity：Experimental observations in the anaesthetized rat．Neurosci，1995．68(4)：1097．
5 Gross PM，Teasdate GM，Angerson WJ，et al．H2 receptor mediate increases in permeability of the blood-brain barrier during arterial histamine infusion．Brain Res，1981，210(3)：396．
6 Globus MYT，Wester P，Busto R，et al．Ischemia-induced extracel lular release of serotonin plays a role in CAl neuronal cell death in rats．Stroke，1992(11)，23：1595．
7 Choi DW，Maulucci-Gedde M，Kriegstein AR．Glutamate neurotxity in cortical cell culture．J Neurosci，1987，7(2)：357．
8 Nichools DG，Sihra TS．Synaptosomes possess and an exocytotic pool of glutamate．Nature，1986，321(26)：772．
9 Maickel RP，Cox RH，Saillant J，et al．A method for the determination of serotonin and norepinephrine in discrete areas of rat brain．Int J Neuropharmacol，1968，7(3)：275．
10 冯亦璞，胡盾，张丽英．丁基苯酞对小鼠全脑缺血的保护作用．药学学报，1995，30(10)：741．
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脚注
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