目的：建立测定人血浆中芬太尼(Fen)浓度的HPLC测定法，为临床Fen的药动学研究提供科学的分析技术。方法：Fen测定以μ-Bondapak-C₁₈为固定相,水(含0.005mol·L^-1.辛烷磺酸钠和0.01mol·L^-1磷酸)乙腈(69∶31)为流动相，以阿芬太尼为内标物，紫外检测波长为220nm。结果：验证实验表明，Fen与内标物分离良好，血浆中Fen的平均回收率为99.97%，日内、日间的RSD值均小于8%。血药浓度在10ng·ml^-1～200ng·ml^-1范围内与峰面积呈线性关系，r=0.9996。本方法最低检测浓度为5ng·ml^-1。结论：本方法具有较高的准确度，方法灵敏，专一性好，适用于临床Fen血药浓度的测定。

OBJECTIVE: To establish a rapid, simple and sensitive HPLC method for determination of fentanyl (Fen) plasma concentration and provide scientific analysis for the studies of the pharmacokinetics of fentanyl in clinical anesthesia. METHOD: Fen was separated on μ-BondapakC₁₈ column with a mobile phase of water (containing 0.005 mol·L^-1 C₈H₁₇O₃Sna and 0.01 mol·L^-1H₃PO₄)acetonitrile (69∶31) and detected at 220 nm. Internal standard was alfentanil. RESULTS: The separation of Fen and alfentanil was proved to be highly satisfactory. The mean recovery of Fen was 99.97%. The intraday and interday RSD were all less than 8%. The assay linearity was determined over the range of 10 ng·ml^-1～200 ng·ml^-1in plasma (r=0.999 6). The minimum detection of Fen was 5 ng·ml^-1. CONCLUSION: This method possesses high accuracy and precision, and is sensitive and specific. The method could be used in the determination of plasma concentration in clinics.