OBJECTIVE: To prepare the poly (DL-lactide) aclacinomycin nanoparticle in order to evaluate the nanoparticle delivery system and to develop the pharmaceutics of aclacinomycin nanoparticle. METHODS: An optimal design was selected to establish the optimal condition, and the interfacial polymerization method was used to prepare the nanoparticle system. RESULTS: The mean diameter of the nanoparticle was 80 nm, the mean drug loading was 18.5%, and the drug embedding ratio was 86.7%. CONCLUSION: The technology of preparation of poly (DL-lactide) aclacinomycin nanoparticle is practicable and successful.