目的：建立血浆中塞利洛尔浓度的高效液相色谱分析方法，并用此方法对正常人po塞利洛尔片进行药动学研究。方法：血浆标本经碱化、乙酸乙酯和硫酸提取，以乙腈-水-磷酸盐缓冲液为流动相，紫外237nm处检测。结果：线性范围10μg·L^-1-5000μg·L^-1，日内RSD为1.70%-4.06%，日间RSD在1.86%-4.03%范围内，方法回收率在(97.78±2.90)%-(102.00±3.10)%范围内，最低检测浓度2μg·L^-1?结论：此方法简便可靠，测定灵敏度高,可用于塞利洛尔临床药动学和药效学研究?

OBJECTIVE: To establish a HPLC method for the determination of celiprolol in human plasma and investigate the pharmacokinetic characteristics of celiprolol in normal subjects after a single oral dose. METHODS: The plasma was extracted with ethyl acetate, sulfuric acid after base purification. The mobile phase was an aqueous solution containing acetonitrile and phosphate buffer. Absorbance of the effluent was monitored at 237 nm. RESULTS: The method was linear within the range of 10 to 5 000 μg·L^-1 and the limit of detection was 2 ng·ml^-1. The average relative diviation (RSD) of within day ranged from 1.70% to 4.06%, and day to day from 1.86% to 4.03%. The recoveries of celiprolol were (97.78±2.90)% to (102.00±3.10)%. CONCLUSION: The method permits characterization of the pharmacokinetics and pharmacodynamics of celiprolol in clinical setting.