摘要
目的：建立羟自由基(·OH)引发的人红细胞膜氧化损伤实验模型，研究橙皮苷对红细胞膜氧化损伤的保护作用。方法：以Fenton反应产生的羟自由基引发人红细胞膜氧化损伤，并以此为实验模型研究橙皮苷对红细胞膜氧化损伤的影响。结果：·OH能引起红细胞膜脂质过氧化，丙二醛(MDA)含量显著升高；膜脂流动性下降以及膜重封闭能力减小。而红细胞膜经一定量橙皮苷预先处理后，膜MDA含量明显减少；膜脂流动性和膜重封闭能力可显著提高。结论：橙皮苷对·OH引起的红细胞膜氧化损伤有一定的保护作用。
Abstract
OBJECTIVE: To establish a model of oxidative damage in erythrocyte ghost induced by hydroxyl free radical, and investigate the influence of hesperidin on oxidative erythrocyte ghost. METHODS: The erythrocyte ghost oxidative damage was induced by hydroxyl free radical which was produced by Fenton reaction. The effect of hesperidin on oxidative erythrocyte ghost was observed by the fluorescent assay, including measurement of the content of malondialdehyde (MDA) and membrane fluidity. RESULTS: Hydroxyl free radical increased the content of MDA, decreased membrane fluidity, alleviated blocking degree, and caused lipid peroxidation of erythrocyte ghost. But hesperidin decreased the content of MDA remarkably, improved the membrane fluidity and blocking degree significantly, and inhibited the lipid peroxidation. CONCLUSION: Hesperidin can prevent lipid peroxidation in erythrocyte ghost damage induced by hydroxyl free radical.
关键词
橙皮苷 /
羟自由基 /
红细胞膜
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Key words
hesperidin /
hydroxyl free radical /
erythrocyte ghost
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王晓玲;秦德安.
橙皮苷对羟自由基引发红细胞膜损伤的影响[J]. 中国药学杂志, 1998, 33(02): 83-85
Wng Xiling.
The effect of hesperidin on oxidative erythrocyte ghost induced by hydroxyl free radical [J]. Chinese Pharmaceutical Journal, 1998, 33(02): 83-85
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参考文献
1 泰德安，苏丹，王晓玲，橙皮苷对羟自由基的清除作用．中国药学杂志，1996，31(7)：396．
2 湖南医学院第一附属医院药剂科．橙皮苷生产工艺的研究．中草药通讯，1974，5：22．
3 Dodge JT，Mitchell C，Hanahan DJ．The preparation and chemical characteristies of hemoglobin/free ghosts of human erythrocytes．Arch Biochem Biophy，1963，100：119．
4 Lowry OH，Rosebrough NJ．Farr AI．et al．Protein measurement with folin phenol reagent．J Biol Chem，1951，193：265．
5 翁玉椿，王荣平，卢咏才，等．细胞和细胞膜内过氧脂质的微量定量．细胞生物学杂志．1985，7(3)：54．
6 何学民，秦德安．超氧自由基对人的红细胞膜流动性的影响．华东师范大学学报，1991．4：54．
7 Steck TL，Kant JA．Preparation of impermeable ghosts and insideout vesicles from humen erythrocyte membranes．Methods Enzymol，1974，31：172．
8 秦德安，何学民，王永江．超氧歧化酶对人红细胞膜的保护作用，生物化学与生物物理进展，1989，16(2)：134．
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脚注
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