目的:确立泽泻中alismol的测定方法。方法:对泽泻中的主成分alismol进行分离鉴定以制备对照品，将泽泻粉末经硅胶柱预处理，洗脱液再薄层层析，进行光密度扫描测定。结果:样品alismol含量为0.021%,RSD=4.1%，在1.5 h内稳定性，回收率103. 8%。结论:本方法灵敏、专属，重现性好，简便易行。
Abstract
OBJECTIVE: To stablish TLC-densitometry of alismol in Rhizoma Alismatis. METHODS: The samples were extracted by CC(hexane - CH2 Cl2 ,1:1)method and subjected to TLC-densitometry ( coloured by silimtungstic acid). RESULTS: The alismol content in Rhizoma Alismatis was 0.021%and RSD was 4.1%.CONCLUSION Alismol was efficiently quantitated by TLC-densitometry.
关键词
泽泻 /
倍半萜 /
薄层光密度扫描
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Key words
Alisma orientalis /
alismol /
TLC-densitometry
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参考文献
1 Kato T, Tomita M, Takigana M. et al, Inhibitory effects and active constituents of Alisma Rhiwmes on vascular contraction induced by high consentranon on KCI. Bull Chem Soc Jpu 1994,67:1394.
2 Yamahara J, Kobayashi G, Iwamoto M. et al, The effect of alismol isolated from Alisma orientalis on experimental hypertensive models in rats. Phytotherapy Research，1989,3:57.
3 Matsuda H, Kobayashi G, Yamahara J. et al, Quantitative Analysis of Alismol in Alismatis Rhizoma. Shoyakugaku Zasshi, 1988, 42: 147.
4 Nakajima Y, Satoh Y, Katsumata M, et al,Terpenoids of Alisma orientate rhizome and the crude drug alismatis rhizoma.Pytochemistry, 1994,36:119.
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脚注
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基金
国家中医药管理局基金项目第950013号
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