目的:从鸡后鳃体分离纯化降钙素并对其性质进行研究。方法:采用丙酮及正丁醇-醋酸-水(75:7.5:21)对后鳃体进行提取,提取物再进行CM - 52柱层析进行分离纯化。结果:提取物经高效液相色谱分析、酶免测定及大鼠血清钙降低实验表明为鸡降钙素。结论:获得了具有一定纯度,并有生物活性的鸡降钙素。
Abstract
OBJECTIVE: To isolate and characterize calcitonin from chicken ultimobranchial glands. METHODS:Chicken ultimobranchial glands were extracted by acetone and butanol-acetic acid-water (75:7.5:21).The extracts were purified by CM-52 column chromotography. RESULTS: The results of HPLC, EIA and rats hypercalcemic assay showed that the extracts are calcitonin. CONCLUSION:The chicken calcitonin, which has been partly purified and has biological activity, was obtained.
关键词
鸡降钙素 /
高效液相色谱法 /
酶免分析 /
大鼠血清钙测定
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Key words
chicken calcitonin /
HPLC /
EIA /
rats hypercalcemic assay
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参考文献
1 Copp DH, Davidson ATG. Evidence for a new paratlyroid hormone which lowers blood calcium. Proc Canad Fecl Biol Soc,1991,4:17.
2 Azria M, The Calcitonins.Basel : Karger.1989 :1~19.
3 刘忠厚,马述仕,薛延,等.骨质琉松症.北京:化学工业出版社,1992:213~214.
4 Wallach S. Effect of salmon calcitonin on skeletal mass in osteoporosis.Curr Ther Rec. 1977,22:556.
5 Nieto A, Moya F, Candela J LR. Iwlation and properties of two calcitonins from chicken nltimohranchial glands. Biochim BioDhvs Acta.1973:383.
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脚注
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基金
国家教委博士点基金项目94-1-3
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