鸡降钙素的分离及性质研究

李元;郭强;王醒;姜蓉;李嗣英;

中国药学杂志 ›› 1997, Vol. 32 ›› Issue (07) : 404-406.

中国药学杂志 ›› 1997, Vol. 32 ›› Issue (07) : 404-406.
生化药物

鸡降钙素的分离及性质研究

  • 李元;郭强;王醒;姜蓉;李嗣英;
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Isolation and characterization study of chicken calcitonin

  • Li Yuan
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摘要

目的:从鸡后鳃体分离纯化降钙素并对其性质进行研究。方法:采用丙酮及正丁醇-醋酸-水(75:7.5:21)对后鳃体进行提取,提取物再进行CM - 52柱层析进行分离纯化。结果:提取物经高效液相色谱分析、酶免测定及大鼠血清钙降低实验表明为鸡降钙素。结论:获得了具有一定纯度,并有生物活性的鸡降钙素。

Abstract

OBJECTIVE: To isolate and characterize calcitonin from chicken ultimobranchial glands. METHODS:Chicken ultimobranchial glands were extracted by acetone and butanol-acetic acid-water (75:7.5:21).The extracts were purified by CM-52 column chromotography. RESULTS: The results of HPLC, EIA and rats hypercalcemic assay showed that the extracts are calcitonin. CONCLUSION:The chicken calcitonin, which has been partly purified and has biological activity, was obtained.

关键词

鸡降钙素 / 高效液相色谱法 / 酶免分析 / 大鼠血清钙测定

Key words

chicken calcitonin / HPLC / EIA / rats hypercalcemic assay

引用本文

导出引用
李元;郭强;王醒;姜蓉;李嗣英;. 鸡降钙素的分离及性质研究[J]. 中国药学杂志, 1997, 32(07): 404-406
Li Yun. Isolation and characterization study of chicken calcitonin[J]. Chinese Pharmaceutical Journal, 1997, 32(07): 404-406

参考文献

1 Copp DH, Davidson ATG. Evidence for a new paratlyroid hormone which lowers blood calcium. Proc Canad Fecl Biol Soc,1991,4:17. 2 Azria M, The Calcitonins.Basel : Karger.1989 :1~19. 3 刘忠厚,马述仕,薛延,等.骨质琉松症.北京:化学工业出版社,1992:213~214. 4 Wallach S. Effect of salmon calcitonin on skeletal mass in osteoporosis.Curr Ther Rec. 1977,22:556. 5 Nieto A, Moya F, Candela J LR. Iwlation and properties of two calcitonins from chicken nltimohranchial glands. Biochim BioDhvs Acta.1973:383.

基金

国家教委博士点基金项目94-1-3

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