目的:探讨氯化血红素提取工艺的最佳条件，并制定其质量标准。方法:以氯化血红素的得率为指标，运用正交试验优选氯化血红素提取工艺的最佳条件，用红外光谱、紫外光谱作定性验证，用紫外光谱法测定氯化血红素 的含量。结果:通过正交试验，得出血红蛋白与冰醋酸的比例1:4、反应温度118℃、反应时间20 min为氯化血红素最佳提取工艺，产品得率高，质量可控。结论:本提取工艺及质量标准可行。

OBJECTIVE: To investigate the best conditions of extraction techniques for hemin and to work out the quality standards of hemin. METHODS: The best conditions of extraction techniques for hemin were optimized with orthogonal experiments, using the yield of hemin as an index. The qualitative analysis with the IR-and UV-spectra and determination of hemin with UV-spectrophotometry was made. RESULTS: The best conditions for the extraction techniques of hemin were the reaction solution containing Hb and glacial acetic acid in the ratio of 1:4 for 20 min at 118℃.The yield of hemin was high. Its quality was controlled. CONCLUSION: The extraction techniques and the quality standards are feasible.