OBJECTIVE: To study antioxidative action of Zingiber officinales petroleum extracts with a four oxygen free radicals system. METHODS: The antioxidative action on erythrocyte with O2 of extracts was studied by spectrophotometry. The protective action on the treatment of erythrocyte membranes with H2O2 and the cell membrane fluidity with fluorescence polarization .were studied. The rate of inhibition and scavenging of·OH generation and the antiaction of lipid peroxidant of zingiber extracts were studied by using fluorescence. RESULTS: The zingiber extracts could significantly antioxidative action on erythrocyte with O2,and had protective action on erythrocyte membranes and caused an increase in fluidity. The extracts possessed great scavenging effect on ·OH, the rate of inhibition and scavenging were 92.41%and 97.39% respectively when c=2.09g·L-1, and the lipid peroxidation of mouse liver mitochondria was markedly inhibited by extracts at c=4.0 g·L-1 .The rate of inhibition was 86.81%.CONCLUSION; The extracts of zingiber significant inhibition and scavenging effect on four oxygen free radicals, and can be used as an antioxidant.
Wng Qio .
Antiactive oxygen effect of Zingiber officinale petroleum extracts with a four oxygen free radicals system[J]. Chinese Pharmaceutical Journal, 1997, 32(06): 343-346