目的:研究血清中青藤碱的液-固净化技术和在家兔体内的动力学特征。方法:自制硅胶萃取柱及萃取仪，将血清中青藤碱净化后，点样于硅胶G-硅胶GF254 ( 2 : I )薄层板上，以氯仿-甲醇(19:2)为展开剂，在岛津CS-930上，用双波长(λs275 nm,λR 320 nm )锯齿法扫描测定。结果:血清中青藤碱的固相提取回收率为96.95%±s 7.59%,用薄层扫描法测绘了2只家兔静注青藤碱后的药物浓度-时间曲线。结论:自制萃取柱净化血清样品简便、有效;青藤碱的薄层扫描测定法灵敏、准确。
Abstract
OBJECTIVE: To study the purification of sinomenine in serum by the liquid-solid extraction and the pharmacokinetics of sinomenine in rabbits. METHODS: To sinomenine in serum was extracted by silica column which made in our laboratory and redissolved in CHC13 .The sample was applied on silica GF254 plate and developed with CHCI3- CH3OH(19:2).The spot was detected by CS-930 atλs=275 m,λR=320 nm. RESULTS: The average recovery was 96.95%±s7.59%for SPE(solid phase extraction) procedure in serum. The drug-time curve of sinomenine in rabbitswas determined. CONCLUSION: The SPE columns which made in our laboratory are simple and efficient for the purification of serum samples. The TLC is sensitive and precise for the determination of sinomenine.
关键词
青藤碱 /
血清 /
薄层扫描测定法
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Key words
smomenme /
serum chromatography
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参考文献
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脚注
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