木蹄多糖及其抗肿瘤活性的研究

韩文志;高其品;姜瑞芝;张大军;刘平

中国药学杂志 ›› 1994, Vol. 29 ›› Issue (S1) : 8-10.

中国药学杂志 ›› 1994, Vol. 29 ›› Issue (S1) : 8-10.
中药及天然药物

木蹄多糖及其抗肿瘤活性的研究

  • 韩文志;高其品;姜瑞芝;张大军;刘平
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Polysaccharides and their anti-tumor activity muti(Pyropolyporus fomentarlus)

  • HAN Wen-zhi1; GAO Qi-pin;JIANG Rui-zhi;ZHANG Da-jun;LIU Ping
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摘要

木蹄[(Pyropolypourus fomentarius(L.ex.Fr.)Teng]子实体热水提取物经乙醇沉淀.乙酸铜分步,DEAE一纤维素柱层析,得到4个多糖组分(BI~BN)。药理实验表明:BI BN部分对优Hela细胞有明显杀伤作用;BI,BI部分对Hela细胞没有杀伤作用,但显著抑制了 Hela细胞生长。水洗脱部分(BI)经 Sepharose 4B凝胶柱层析、Sep-PakC柱层析,得到两种纯多糖 BI-IW,BI-IE。分别测定了它们中蛋白质、糖醛酸、总糖含量及分子量,并进行了组成糖分析。

Abstract

Four polysaccharldes(BI—BN)were extracted from Mutl[Pyropolyporus fomen- tarius(L.ex Fr.)Teng] by water and precipitated with ETOH.Ion-exchange chormatography was done on DEAE-cellouse.The pharmacological test showed that BⅢ and BⅣkilling activity to Hela cell.BI an BⅡ showed no influence on Hela cell,but BI and BⅡ obviousg inhibited growth of Hela cells.Two homogeneous polysaccharides BI-IW.BI-IE were isolated from BI by chromatography on sepharse 4B,Sep-pak C18 column.The contents of protein·uronic acid and carbohydrate were detected and their component sugars were analysed.

关键词

木蹄 / 多糖 / 组成糖 / 抗癌作用

Key words

polysaccharides / component sugar / anti-tumor activity

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导出引用
韩文志;高其品;姜瑞芝;张大军;刘平. 木蹄多糖及其抗肿瘤活性的研究[J]. 中国药学杂志, 1994, 29(S1): 8-10
HN Wen-zhi;GO Qi-pin;JING Rui-zhi;ZHNG D-jun;LIU Ping. Polysaccharides and their anti-tumor activity muti(Pyropolyporus fomentarlus)[J]. Chinese Pharmaceutical Journal, 1994, 29(S1): 8-10

参考文献

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