用高效液相色谱法测定维司力农，以甲醇－1mmol／L醋酸（50：50）为流动相，检测波长271nm。最低检出全为0．05ng（R／N，5：1），在供试溶液为1～200μg／ml范围内峰面积与浓度线性关系良好（r＝0．9999），重现性好，RSD为0．74％，回收率为99．00％±0．29％（n＝9）。本法可同时分离维司力在及其合成中间体，并用于考察维司力农的微量有关杂质。
Abstract
Vesnarinone,a new positive inotropic agent,was synthesized by Tominaga et al ofthe Otsuka Pharmaceutical Co. Ltd. Institute in 1982. Vesnarinone and its impurities by reversed-phase HPLC were determined chromatograapy consists of a Shim-pack CLC ODS column(150mmx6mmID)with methanol :1mmol/L acetic acid(50: 50)as the mobile phase at a flow rate of 0.8ml/min. Spectrophotomtric detection was at 271nm and column temperature at 25℃. The detec-tion limits of vesnarinone was 0. 05ng(R/N,5:1),and the calibration curve was linear(r=0.99998)in the concentration range of 1～200Ug/ml.RSD was 0.74%.The recovery was 99.00%±0.29%（n=9）.Vesnarinone and its intermediate compounds could be separated well under this coudition.The assay of vesmarinone and the related impurities was developed.
关键词
高效液相色谱法 /
维司力农
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Key words
HPLC /
vesnarinone
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参考文献
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