摘要
采用正交函数、导数光谱法分别测定复方土荆皮酊中苯甲醚、水杨酸、土荆皮含量。苯甲酸用正交函数法在220~230nm区间,△λ=2nm,6个波长,支测定,计算P2值,以含量对P2值作线性回归,r=0.9986;水杨酸在265~330nm区间作一阶导数光语,△λ=1nm,在309nm处以含量对谷极值作线性回归,r=0.9995;土荆皮在360nm处测定,其浓度正比于吸收值,作线性回归,r=0.9982。3个组分回收率±RSD依次为99.25%±1.12%,99.39%±0.44%,99.25%±1.00%。
Abstract
We proposed a method of determination for benzoic acid,salicylic acid and Pseu-dolaricis, in tincturae cortex Pseudolaricis Co.Benzoic acid was measured at 220~ 230nm ,at 2nmintervals,using 6-point quadratic orthogonal polynomial to calculate P_2. Linear regression was ob-tained for the content vs P_2 value,r=0. 9986. Salicylic acid was measured by l-order derivativespectrum,at 265~330nm. linear regession was obtaied for the content vs valley depth,r=0. 9995.The content of pseudolaricis was measured by the absorbance at 360nm.Its concentration was proportional to absorbance,with r=0.9982 for the linear regression.The mean recoveries(±RSD) of the three ingredients were 99.25%±1.22%,99.39%±0.44%,99.25%±1.00%,respectively.
关键词
正文函数法 /
导数光谱法 /
复方土荆皮酊
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Key words
orthogonal function /
derivative spectrum /
tinctura cortex Pseudolaricis composita
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翁信源;曹风兰;李惠珍.
复方土荆皮酊中3个组分的含量测定[J]. 中国药学杂志, 1994, 29(10): 620-623
WENG Xin-yun;CO Feng-In;LI Hui-zhen.
Determination of three ingredients in tincturae cortex Pseudolaricis compositae[J]. Chinese Pharmaceutical Journal, 1994, 29(10): 620-623
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参考文献
1中国人民解放军总勤部卫生部编.中国人民解放军医疗单位制剂规范,1991:121.
2相秉仁,计算药学。北京:中国医药科技出版社,1990:136-140
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脚注
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