酶电极法测定葡萄糖注射液中葡萄糖含量的研究

孙士青;王宁;何伟;胡春玲

中国药学杂志 ›› 1994, Vol. 29 ›› Issue (09) : 546-548.

中国药学杂志
中国药学杂志 ›› 1994, Vol. 29 ›› Issue (09) : 546-548.
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酶电极法测定葡萄糖注射液中葡萄糖含量的研究

  • 孙士青；王宁；何伟；胡春玲
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Enzyme electrode for the determination of glucose in injection

  • SUN Shi-qing;Wang Ning1;HE Wei2;HU Chun-ling3
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摘要

以固定化葡萄糖氧化酶和h2H2电极构成流型葡萄糖酶电极生物传感器，研究了葡萄糖生物传感器分析法的分析条件、各种参数，并以旋光法测定葡萄糖注射液的含量进行对比，获得较为一致的结果。

Abstract

Glucose oxidase was ilnmobilized at a platinized activated carbon electrode and theenzyme electrode was used for the amperomet ric determination of glucose in injection by theelectrochemical detection of enzymically prod uced hydrogen peroxide. The performance of theelectrode was cornpared with the traditionaltechnique optical rotation.

关键词

生物传感器 / 葡萄糖氧化酶 / 酶电极 / 葡萄糖测定

Key words

biosensor / glucose oxidase / enzyme electrode / glucose analysis

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孙士青;王宁;何伟;胡春玲. 酶电极法测定葡萄糖注射液中葡萄糖含量的研究[J]. 中国药学杂志, 1994, 29(09): 546-548
SUN Shi-qing;Wng Ning;HE Wei;HU Chun-ling. Enzyme electrode for the determination of glucose in injection[J]. Chinese Pharmaceutical Journal, 1994, 29(09): 546-548

参考文献

1Clark LC, Lyons C. The Hydrogen peroxide sensing plantinum anodes as an analytical enzyme electrode. Ann NY Acad 1962,29. 2Ives DJC,Janz GJ. Reference electrodes. In： Karubel ed.Theory and Practice. Vol. 1. New York ： Academic Press,1961:179-183. 3Clark LC, Clark EW. Oxygen electrode. In： Bicher HI.Oxygen transport to tissue. Plenum, New York： 1973 :127-133. 4Silver I A. Enzyme electrode. In： Kessler M. Ion and Enzyme Electrodes in Biology and Medicine. Munich：Urban Schwarzenberg, 1976:189-332. 5张先恩编.生物电极、生物传感技术原理与应用.长春：吉林科学技术出版社,1991:55-72

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