采用HPLC测定血清中环丙氟哌酸的浓度。流动相为14％甲醇，8％乙腈，78％磷酸盐缓冲液（其中含0．3％氢氧化四丁基铵，0．02mol／L磷酯二氢钾，pH＝3．0±0．02）。采用反相色谱柱，277nm紫外检测。二氯甲烷提取，以二氟沙星为内标物，血样中药物检测限是10ng／ml，回收率98．8％，日内相对标准偏差是3．5％，日间相对标准偏差是1．38％。通过兼用HPLC和生物检定法测定健康受试者的血清药物浓度，对两种分析方法进行比较，将用两种方法测定的结果（n＝80）进行线性回归，直线的斜率是0．91，相关系数是0．959（P＜0．01）。

A simple accurate and reproducible high performance liquid chromatographic proce-dure was developed for the determination of ciprofloxacin(CIP)in serum。 The mobile phase con-tained 14%methanol , 8%acetonitrile and 78%phosphate buffer solution(0.3%tetrabutylam-moinium hydroxide,0. 02mol/L potassium dihydrogenphosphate, pH=3.0±0. 02 phase analyti-cal column, with ultraviolet detection).This method involved a simple one-step extraction proce-dure of dichloromethane for CIP in serum, and utilized difloxacin as internal standard. The de-tection limit for blood level wsa 10ng/ml. The mean recovery of CIP in the method was 98.8%。The ralative standard deviation was 3.5%for within day precision and 1.38%for day to day pre-cision。 High performance liquid chromatography and the bioassay procedure were comparedthrough assay of a series of actual volunteers′s specimens (n=80).The pregression of the bioassayand high pertformance liquid chromatographic assay data gave a linear relationship with a slope of0.91 and a correlation coefficient of 0.959(P<0.01)。