目的 对比研究大花红景天(Rhodiola crenulata)和长鞭红景天(Rhodiola fastigiata)多糖的结构特征与Toll样受体4(toll like receptor,TLR4)激动活性差异。方法 采用水提醇沉法提取大花红景天和长鞭红景天的多糖成分,分离纯化分别得到了大花红景天多糖(Rhodiola crenulata polysaccharide, RCP)、长鞭红景天多糖(Rhodiola fastigiata polysaccharide,RFP)。并采用UV、IR、HPGPC、GC-MS等波谱方法分析RCP和RFP的多糖组成成分和结构;在HEK-Blue hTLR4细胞上对比RCP和RFP的TLR4激活率。结果 RCP为均一多糖,相对分子质量约为125 000,含阿拉伯糖和半乳糖,摩尔比为0.089:2.233;RFP为杂多糖,相对分子质量约为68 000/134 000,含鼠李糖、阿拉伯糖、半乳糖,摩尔比为0.646:1.148:2.206。在100 μg·mL-1的作用浓度下,RCP的TLR4激动率为87.59%,RFP的TLR4激动率为33.39%。结论 RCP组分较RFP更加均一,TLR激动活性更强,为红景天属多糖成分的研究与开发提供参考。
Abstract
OBJECTIVE To compare the structural characteristics and the difference in toll like receptor(TLR4) agonistic activity of Rhodiola crenulata and Rhodiola fastigiata polysaccharides. METHODS Rhodiola crenulata polysaccharide (RCP) and Rhodiola fastigiata polysaccharide (RFP) were isolated and purified by water extraction and alcohol precipitation. UV, IR, HPGPC, GC-MS and other spectral methods were used to analyze the composition and structure of RCP and RFP polysaccharides; HEK-Blue hTLR4 cells were used to compare the TLR4 activation rate of RCP and RFP. RESULTS RCP is a homogeneous polysaccharide with a molecular weight of about 125 000, containing arabinose and galactose, with a molar ratio of 0.089:2.233; RFP is a heteropolysaccharide with a molecular weight of about 68 000/134 000, containing rhamnose, arabinose, and galactose, with a molar ratio of 0.646:1.148:2.206. At the concentration of 100 μg·mL-1, the TLR4 activation rate of RCP was 87.59%, and the TLR4 activation rate of RFP was 33.39%. CONCLUSION RCP components are more homogeneous than RFP, and TLR agonistic activity is stronger, which provides a reference for the research and development of Rhodiola polysaccharide components.
关键词
大花红景天 /
长鞭红景天 /
结构表征 /
Toll样受体4
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Key words
Rhodiola crenulata /
Rhodiola fastigiata /
structural characteristics /
TLR4
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参考文献
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脚注
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基金
浙江省临床肿瘤药理与毒理学研究重点实验室项目资助(2020E10021);杭州市农业与社会发展科研计划项目资助(20172016A01)
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