Abstract：OBJECTIVE To verify a new reference standard for N-glycyl-L-tyrosine in order to assure the quality of amino acids (15) and dipeptides (2) injection. METHODS UV, HRMS,1H-NMR,13C-NMR and 2D NMR were used to confirm the structure, and the content of the product was calculated by mass balance method, which was also confirmed by titrimetric method. RESULTS The structure of this product was identified and the content of N-glycyl-L-tyrosine was assigned as 88.4%. CONCLUSION According to the physicochemical properties of the compound, were used multiple methods for the qualitative and quatitative analysis to ensure the calibration results of the national reference standard accurately. The first established N-glycyl-L-tyrosine national reference can be efficiently applied for the assay of amino acids (15) and dipeptides (2) injection.
刘博, 任丽萍, 张佟, 黄姗, 范慧红. 首批甘氨酰-L-酪氨酸国家对照品的标定研究[J]. 中国药学杂志, 2020, 55(22): 1890-1894.
LIU Bo, REN Li-ping, ZHANG Tong, HUANG Shan, FAN Hui-hong. Verification of National Reference Standard of N-glycyl-L-tyrosine. Chinese Pharmaceutical Journal, 2020, 55(22): 1890-1894.
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2020, Vol. 55 
